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La unión de la proteína priónica asociada a la enfermedad con el plasminógeno

M B Fischer¹, C Roeckl, P Parizek

¹Institute for Neuropatholgy, University Hospital of Zurich, Switzerland.

|December 2, 2000

Resumen

Los investigadores identificaron plasminógeno en la sangre que se une a la proteína priónica asociada a la enfermedad (PrPSc) pero no a la proteína priónica normal (PrPC). Este descubrimiento podría ayudar en el diagnóstico de las encefalopatías espongiformes transmisibles.

Área de la Ciencia:

La neurociencia es la neurociencia.
La bioquímica es la bioquímica.
Biología Molecular Biología Molecular

Sus antecedentes:

Las encefalopatías espongiformes transmisibles (ETS) están relacionadas con la acumulación de proteína priónica anormal (PrPSc), una forma mal plegada de la proteína priónica celular (PrPC).
El mecanismo de la replicación de PrPSc y su interacción con los componentes celulares para causar neuropatología sigue siendo incompletamente entendido.
Distinguir entre PrPC y PrPSc basado en la conformación ha sido un desafío significativo.

Objetivo del estudio:

Para identificar los factores endógenos en la sangre que pueden discriminar entre PrPC y PrPSc.
Investigar el papel de tales factores en la patogénesis de la enfermedad priónica y las posibles aplicaciones de diagnóstico.

Principales métodos:

Cribado de sangre humana y de ratón para actividades que se unen a PrPSc pero no a PrPC.
La identificación de la proteína específica responsable de esta actividad de unión.
Caracterización de la interacción de unión mediante ensayos bioquímicos, incluida la interrupción con denaturantes y estudios de competencia.

Principales resultados:

Identificó el plasminógeno, una pro-proteasa sanguínea, como una proteína que se une a PrPSc y la infectividad priónica, pero no a PrPC.
Se demostró que la unión de PrPSc al plasminógeno es dependiente de la conformación, siendo abolida por la urea 6M o la guanidina.
Se demostró que el sitio de unión a la lisina 1 del plasminógeno (kringles I-III) media esta unión específica, que puede competir con la lisina.

Conclusiones:

El plasminógeno es el primer factor endógeno identificado que se une selectivamente al conformador PrPSc patológico sobre el PrPC normal.
Esta propiedad de unión única del plasminógeno ofrece una vía potencial para el desarrollo de herramientas de diagnóstico para las EET.

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