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Factores de transcripción de Arabidopsis: análisis comparativo de todo el genoma entre eucariotas
J L Riechmann1, J Heard, G Martin
1Mendel Biotechnology, 21375 Cabot Boulevard, Hayward, CA 94545, USA. jriechmann@mendelbio.com
Science (New York, N.Y.)
|December 16, 2000
Resumen
Arabidopsis thaliana posee más de 1500 factores de transcripción, con muchas familias específicas de plantas. El análisis comparativo revela divergencias evolutivas en la regulación de la transcripción en los eucariotas, destacando las combinaciones de dominios de unión al ADN específicos del linaje.
Área de la Ciencia:
- La genómica comparada es una genómica comparada.
- Evolución molecular de la evolución molecular.
- Familias de factores de transcripción.
Sus antecedentes:
- La secuenciación del genoma de la Arabidopsis thaliana proporciona una base para análisis comparativos a gran escala.
- Los factores de transcripción (TF) son reguladores cruciales de la expresión génica, que controlan los procesos biológicos fundamentales.
- Comprender la evolución de TF ofrece información sobre la diversificación de la vida.
Objetivo del estudio:
- Para llevar a cabo un análisis comparativo de los reguladores transcripcionales en Arabidopsis thaliana frente a los de otros reinos eucariotas.
- Investigar los orígenes evolutivos y la diversificación de las familias de factores de transcripción.
- Explorar la conservación y la divergencia de los dominios de unión al ADN y sus disposiciones.
Principales métodos:
- Identificación y clasificación de todo el genoma de los factores de transcripción en Arabidopsis thaliana.
- Análisis comparativo de las familias de Arabidopsis TF con ortólogos en otros linajes eucariotas.
- Análisis bioinformático de dominios de unión al ADN conservados y sus patrones combinatorios.
Principales resultados:
- Arabidopsis thaliana codifica más de 1500 factores de transcripción, con aproximadamente el 45% pertenecientes a familias específicas de las plantas.
- Los TF comunes a todos los eucariotas muestran una similitud limitada fuera de los dominios de unión al ADN conservados.
- Se observaron importantes disposiciones específicas de linaje de dominios de unión al ADN conservados.
Conclusiones:
- El genoma de la Arabidopsis thaliana alberga un repertorio sustancial de factores de transcripción, incluidos numerosos factores específicos de las plantas.
- Los procesos evolutivos han generado diversidad en la regulación transcripcional, particularmente a través del uso combinatorio de dominios conservados.
- La genómica comparativa revela distintas trayectorias evolutivas en las familias de factores de transcripción a través de los reinos eucariotas.
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