Video Experimental Relacionado
Updated: Jul 17, 2026
06:40
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
El silenciamiento independiente de la replicación del ADN en S. cerevisiae
1Division of Genetics and Development, Department of Molecular and Cell Biology, University of California, 401 Barker Hall, Berkeley, CA 94720, USA.
Science (New York, N.Y.)
|February 7, 2001
Resumen
La replicación del ADN no es necesaria para silenciar los loci de tipo de apareamiento en Saccharomyces cerevisiae. La formación y el silenciamiento de la heterocromatina ocurren independientemente del tenedor de replicación.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética de la levadura Genética de la levadura
- La epigenética es la epigenética.
Sus antecedentes:
- Los loci de apareamiento silencioso en Saccharomyces cerevisiae son reprimidos a través de la formación de heterocromatina.
- El ensamblaje de la heterocromatina requiere un evento del ciclo celular entre la fase S temprana y la fase G(2) / M.
- La replicación del ADN ha sido el presunto desencadenante de este silenciamiento dependiente del ciclo celular.
Objetivo del estudio:
- Investigar si la replicación del ADN a través del locus tipo de apareamiento silencioso HMRa es esencial para establecer el silenciamiento mediado por heterocromatina.
- Para diferenciar el papel de la replicación del ADN de otros eventos del ciclo celular en el establecimiento de silenciamiento.
Principales métodos:
- Monitoreo de la formación de heterocromatina en el locus cromosómico HMRa.
- Monitoreo de la formación de heterocromatina en una cinta de HMRa extracromosómica no replicante.
- Analizando el establecimiento de silenciamiento a medida que las células progresaban a través de la fase S.
Principales resultados:
- El silenciamiento se estableció con la misma eficiencia tanto en los loci HMRa cromosómicos como extracromosómicos.
- La formación y el silenciamiento de la heterocromatina ocurrieron independientemente del paso del tenedor de replicación del ADN a través del locus HMR.
- El establecimiento de silenciamiento mantuvo una dependencia del ciclo celular, a pesar de la ausencia de replicación.
Conclusiones:
- La suposición prevaleciente de que se requiere la replicación del ADN para el silenciamiento de los loci de apareamiento en la levadura es incorrecta.
- El establecimiento de silenciamiento dependiente del ciclo celular puede ocurrir sin el paso de la horquilla de replicación a través del locus objetivo.
- Otros eventos del ciclo celular, no la replicación del ADN en sí, probablemente regulan el momento de la formación y silenciamiento de la heterocromatina.
Videos de Conceptos Relacionados
02:35Replication in Prokaryotes
Overview
02:40Chromosome Structure
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
02:35Replication in Prokaryotes
Overview
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
01:32Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.