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Los transitorios de calcio filopodial promueven el giro del cono de crecimiento dependiente del sustrato

T M Gomez¹, E Robles, M Poo

¹Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093, USA. tmgomez@facstaff.wisc.edu

Science (New York, N.Y.)

|March 10, 2001

Resumen

Los conos de crecimiento neuronal usan filopodia para sentir el entorno. Estos filopodios generan transitorios de calcio que envían señales al cono de crecimiento, influyendo en el movimiento y la orientación celular.

Área de la Ciencia:

La neurociencia es la neurociencia.
Biología celular Biología celular.
La bioquímica es la bioquímica.

Sus antecedentes:

Los conos de crecimiento neuronal navegan por entornos complejos utilizando filopodia para detectar señales de guía.
Los mecanismos de señalización precisos por los cuales los filopodios comunican información al cono de crecimiento siguen siendo en gran medida desconocidos.

Objetivo del estudio:

Para investigar el papel de señalización de filopodia en la navegación del cono de crecimiento neuronal.
Para aclarar la naturaleza y el impacto de las señales generadas por filopodia.

Principales métodos:

Imágenes en vivo de conos de crecimiento neuronal y filopodios.
Medición de la dinámica intracelular del calcio ([Ca2+]i) mediante el uso de indicadores de calcio.
Manipulación de las interacciones del sustrato y la actividad de los receptores de integrina.

Principales resultados:

Los Filopodia generan elevaciones de calcio intracelular ([Ca2+]i) localizadas y transitorias que se propagan hasta el cono de crecimiento.
Los transitorios de calcio filopodial son dependientes del sustrato e influenciados por los canales de calcio activados por los receptores de integrina.
Estos transitorios de calcio modulan la motilidad filopodial, afectando la velocidad de crecimiento de las neuritas y el comportamiento de giro del cono de crecimiento.

Conclusiones:

Los transitorios de calcio filopodial actúan como señales rápidas y codificadas en frecuencia que regulan la motilidad y la orientación del cono de crecimiento.
Este mecanismo de señalización puede ser un proceso común en varias células móviles, no limitado a las neuronas.

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