Video Experimental Relacionado

Updated: Dec 22, 2025

A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting

A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting

Published on: January 12, 2018

La huella molecular de la señalización gamma del interferón en la angina inestable

G Liuzzo¹, A N Vallejo, S L Kopecky

¹Department of Medicine, Mayo Clinic and Foundation, Rochester, Minnesota, USA.

|March 21, 2001

Resumen

Los monocitos en pacientes con angina inestable muestran signos de activación del interferón-gamma (IFN-gamma), indicado por la translocación nuclear STAT-1 y el aumento de la expresión génica CD64 e IP-10. Esto sugiere que el IFN-gamma juega un papel en los síndromes coronarios agudos.

Área de la Ciencia:

Inmunología Inmunología.
Cardiología Cardiología.
Biología Molecular Biología Molecular

Sus antecedentes:

La activación de monocitos en los síndromes coronarios agudos (SCA) puede involucrar productos bacterianos o citoquinas como el interferón-gama (IFN-gama).
IFN-gamma desencadena la fosforilación STAT-1 y la translocación nuclear, iniciando la inducción de genes.
Este estudio investiga si la IFN-gamma impulsa la activación de los monocitos en la angina inestable (UA) frente a la angina estable (SA).

Objetivo del estudio:

Para determinar si la activación de monocitos en la angina inestable es impulsada por el interferón-gamma (IFN-gamma).
Para comparar la translocación nuclear STAT-1 y la expresión génica inducible por IFN-gamma (CD64, IP-10) en pacientes con UA y SA.

Principales métodos:

Analizando las células mononucleares de la sangre periférica de pacientes con UA y SA.
Cuantificación de la expresión de CD64 en los monocitos CD14 ((+)) a través de la tinción.
Evaluación de la transcripción génica IP-10 mediante PCR.
Evaluación de la translocación nuclear del complejo STAT-1 en extractos nucleares de monocitos.

Principales resultados:

La expresión de CD64 fue significativamente mayor en pacientes con UA.
Los monocitos de pacientes con UA mostraron una respuesta acelerada del gen CD64 al IFN-gamma in vitro.
Se detectaron secuencias de genes IP-10 en el 82% de los pacientes con UA frente al 15% de los pacientes con SA (P<0,001).
Se encontraron complejos STAT-1 en extractos nucleares de monocitos de pacientes con UA, lo que indica in vivo la señalización IFN-gamma.

Conclusiones:

Los monocitos de pacientes con UA muestran marcadores moleculares de la activación reciente de IFN-gamma.
La upregulación de CD64 e IP-10, junto con la translocación nuclear STAT-1, sugiere que IFN-gamma activa parcialmente los monocitos en UA.
El IFN-gamma puede originarse en linfocitos T estimulados, lo que implica respuestas inmunes en la patogénesis del SCA.

Más Videos Relacionados

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Published on: November 20, 2015

Magnetically-Assisted Remote Controlled Microcatheter Tip Deflection under Magnetic Resonance Imaging

Magnetically-Assisted Remote Controlled Microcatheter Tip Deflection under Magnetic Resonance Imaging

Published on: April 4, 2013

Videos de Conceptos Relacionados

Pioneering Patient-Specific Approaches for Precision Surgery Using Imaging and Virtual Reality

Pioneering Patient-Specific Approaches for Precision Surgery Using Imaging and Virtual Reality

Advancements in endovascular treatment have replaced complex open surgical procedures with minimally invasive options, like valve replacement and aneurysm repair. This paper proposes using three-dimensional (3D) modeling and virtual reality to aid in C-arm positioning, angle measurements, and roadmap generation for neuro-interventional catheterization lab procedural planning, minimizing procedure time.

The Transition to an Anterior-Based Muscle Sparing Approach Improves Early Postoperative Function but is Associated with a Learning Curve

The Transition to an Anterior-Based Muscle Sparing Approach Improves Early Postoperative Function but is Associated with a Learning Curve

The anterior-based muscle-sparing approach for total hip arthroplasty (THA) is associated with a learning curve. However, the improved clinical outcome in the early post-operative phase makes the consideration to transition worthwhile.

A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting

A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting

This manuscript describes the Research Prioritization by Affected Communities (RPAC) protocol and findings from its use with women at risk for preterm birth. Using the protocol, women identified and prioritized their unanswered questions about pregnancy, birth and neonatal care aimed at influencing research priority setting by funders and...

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats

Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS...

Magnetically-Assisted Remote Controlled Microcatheter Tip Deflection under Magnetic Resonance Imaging

Magnetically-Assisted Remote Controlled Microcatheter Tip Deflection under Magnetic Resonance Imaging

Current applied to an endovascular microcatheter with microcoil tip made by laser lathe lithography can achieve controllable deflections under magnetic resonance (MR) guidance, which may improve speed and efficacy of navigation of vasculature during various endovascular...

Mouse Models of Periventricular Leukomalacia

Mouse Models of Periventricular Leukomalacia

We established mouse models of periventricular leukomalacia (PVL), the predominant brain injury in premature infants characterized by periventricular white matter lesions. Hypoxia/ischemia with/without systemic infection are the primary causes of PVL. Unilateral carotid ligation and hypoxia exposure with/without lipopolysaccharide injection creates PVL-like lesions in P6...