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El receptor EGF y la señalización Notch actúan aguas arriba de Eyeless/Pax6 para controlar la especificación del ojo
1Department of Cell Biology, Emory University School of Medicine, 1648 Pierce Drive, Atlanta, GA 30322, USA.
Cell
|March 21, 2001
Resumen
Los genes maestros de control para el desarrollo del ojo de Drosophila no se coexpresan temprano. En cambio, las vías de señalización EGF Receptor y Notch inician la especificación ocular más tarde, durante la segunda etapa larval.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología molecular La biología molecular.
Sus antecedentes:
- El ojo compuesto de Drosophila se desarrolla a través de la acción de siete factores nucleares, incluido Eyeless / Pax6, denominados proteínas de control maestro.
- Se sabe que estos factores inducen el desarrollo ocular cuando se expresan ectópicamente y causan pérdida ocular cuando mutan.
- La comprensión previa sugería que estos genes se coexpresaban en el ojo presuntivo embrionario.
Objetivo del estudio:
- Para investigar el tiempo preciso y la coexpresión celular de los genes de especificación ocular en Drosophila.
- Identificar los reguladores aguas arriba de los genes de control maestros involucrados en el desarrollo del ojo.
- Para determinar cuándo ocurre realmente la especificación ocular durante el desarrollo de la Drosophila.
Principales métodos:
- Análisis genético de los patrones de expresión génica.
- Investigando las funciones de las vías de señalización EGF Receptor y Notch.
- Estudios de pérdida de función y expresión ectópica de genes clave del desarrollo.
Principales resultados:
- Varios genes claves de especificación ocular no se coexpresan en las mismas células embrionarias o en el ojo presunto.
- El receptor del factor de crecimiento epidérmico (EGF) y las vías de señalización de Notch funcionan aguas arriba de los genes de especificación ocular.
- Se ha demostrado que la especificación ocular ocurre en la segunda etapa larval, no durante el desarrollo embrionario.
Conclusiones:
- La especificación del ojo en Drosophila es un proceso posterior a lo que se creía anteriormente.
- Las vías de señalización EGF Receptor y Notch juegan un papel homeótico crucial en el inicio del desarrollo del ojo.
- Los patrones de expresión espacial y temporal de los genes de especificación ocular son más complejos que el modelo de control maestro sugerido inicialmente.
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