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Las señales WNT controlan la iniciación de la extremidad dependiente de FGF y la inducción de AER en el embrión de
Y Kawakami1, J Capdevila, D Büscher
1The Salk Institute for Biological Studies, Gene Expression Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Cell
|April 6, 2001
Resumen
Tres vías de señalización WNT regulan la señalización del factor de crecimiento de los fibroblastos (FGF), controlando el desarrollo de las extremidades y la formación de la cresta ectodérmica apical (AER) en embriones de vertebrados.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- Los factores de crecimiento de los fibroblastos (FGF), específicamente FGF-8 y FGF-10, son cruciales para la iniciación de las extremidades y el desarrollo de la cresta ectodérmica apical (AER).
- Comprender los reguladores aguas arriba de la señalización FGF es esencial para comprender el patrón de las extremidades embrionarias.
Objetivo del estudio:
- Investigar el papel de las vías de señalización WNT en la regulación del bucle regulador FGF-8/FGF-10.
- Para identificar factores específicos de WNT involucrados en la iniciación de las extremidades y la inducción de AER.
Principales métodos:
- Análisis de la expresión génica de los factores WNT en embriones de pollo.
- Ensayos funcionales que involucran la expresión génica y la formación de extremidades ectópicas tras la implantación del factor WNT.
Principales resultados:
- Wnt-2b se expresa en el mesodermo de placas intermedias y laterales, induciendo Fgf-10 y la formación de extremidades ectópicas.
- Wnt-8c regula Fgf-10 en la región de las extremidades posteriores y también puede inducir las extremidades ectópicas.
- FGF-10 induce la expresión de Fgf-8 a través de Wnt-3a en el ectoderma de las extremidades.
Conclusiones:
- Tres vías de señalización WNT, mediadas por la beta-catenina, son reguladores clave del bucle FGF-8/FGF-10.
- Estas señales WNT orquestan tanto la iniciación de las extremidades como la inducción de AER durante el desarrollo embrionario de los vertebrados.
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