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La señalización de la proteína G a través de las proteínas tubby
S Santagata1, T J Boggon, C L Baird
1Ruttenberg Cancer Center, Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine of New York University, 1425 Madison Avenue New York, NY 10029, USA.
Science (New York, N.Y.)
|May 26, 2001
Resumen
La disfunción de la proteína de la tina causa obesidad. Tubby actúa como un regulador de transcripción ligado a la membrana, moviéndose al núcleo después de la señalización de la proteína G, vinculando la transducción de señales a la expresión génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La disfunción de la proteína de la trompa está relacionada con la obesidad en ratones.
- El mecanismo molecular preciso de la función de tubby como regulador de la transcripción no se entiende completamente.
Objetivo del estudio:
- Para dilucidar el mecanismo molecular de la función de la proteína tubby en la transducción de señales.
- Para investigar el papel de tubby en las vías de señalización de los receptores acoplados a proteínas G.
Principales métodos:
- Investigó la localización de la proteína tubby y la interacción con el fosfatidilinositol 4,5-bisfosfato utilizando cristalografía de rayos X.
- Se examinó el efecto de la activación de la proteína G alphaq (Galphaq) y la fosfolipasa C-beta en la translocación de tubby.
- Estudió la regulación de la localización de la proteína tipo tubo 3 (TULP3).
Principales resultados:
- Tubby se une con el fosfatidilinositol 4,5-bisfosfato a la membrana plasmática a través de su dominio tubby.
- La activación de Galphaq libera tubby de la membrana, lo que desencadena la translocación nuclear.
- Los dominios Tubby actúan como factores de unión fosforilados-fosfatidil-inositol.
- La localización de TULP3 está regulada de manera similar a tubby.
Conclusiones:
- Las proteínas de la trompa funcionan como reguladores de la transcripción unidos a la membrana.
- La translocación nuclear de tubby se desencadena por la hidrólisis de fosfoinosítidos, que une la señalización de la proteína G con la regulación de la expresión génica.
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