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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
Emi1 es un regulador mitótico que interactúa con Cdc20 e inhibe la anafase que promueve el complejo
J D Reimann1, E Freed, J Y Hsu
1Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA.
Cell
|June 8, 2001
Resumen
Descubrimos Emi1, un inhibidor mitótico temprano que impide la anafase prematura que promueve la activación del complejo / ciclosoma (APC). Esta regulación es crucial para la progresión oportuna del ciclo celular durante la mitosis.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- La mitosis es un proceso crítico de división celular regulado por una compleja maquinaria molecular.
- El complejo promotor de anafase / ciclosoma (APC) es una ligasa ubiquitina E3 clave que controla la progresión del ciclo celular.
- La desregulación de la progresión mitótica puede conducir a graves defectos celulares y enfermedades.
Objetivo del estudio:
- Identificar y caracterizar nuevos reguladores de la mitosis temprana.
- Para aclarar el mecanismo por el cual Emi1 controla la actividad de APC.
- Comprender el papel de Emi1 en la progresión del ciclo celular.
Principales métodos:
- Los extractos de huevo de Xenopus se utilizaron para estudiar la regulación del ciclo celular.
- Se emplearon inmunodepresión y expresión de Emi1 no degradable.
- Se realizaron ensayos de coinmunoprecipitación para estudiar las interacciones de las proteínas.
Principales resultados:
- Emi1, una proteína de la caja F, inhibe la actividad de APC a través de su dominio de unión al zinc.
- Emi1 se acumula antes de la mitosis y se degrada durante la mitosis independientemente de APC.
- El agotamiento de Emi1 retrasa la entrada mitótica, mientras que la Emi1 no degradable causa un bloqueo mitótico al estabilizar los sustratos de APC.
- Emi1 interactúa con Cdc20, un activador de APC, y Cdc20 puede anular la inhibición inducida por Emi1 de la destrucción de la ciclina B.
Conclusiones:
- Emi1 actúa como un inhibidor mitótico temprano al prevenir la activación prematura de la APC.
- Emi1 es un regulador crítico del tiempo de la progresión mitótica.
- La función Emi1 proporciona información sobre el retraso entre la activación de la ciclina B/Cdc2 y la destrucción de la ciclina B en la mitosis.
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