Video Experimental Relacionado
Updated: Jun 26, 2026
08:10
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
Published on: December 14, 2015
Una forma libremente difusible de Sonic hedgehog media en la señalización de largo alcance
1Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, Ohio 45267, USA.
Nature
|June 8, 2001
Resumen
Los investigadores descubrieron una forma libremente difusible de Sonic hedgehog (Shh), llamada s-ShhNp, crucial para la señalización de largo alcance en el desarrollo. Su disponibilidad está regulada por antagonistas de la vía, y forma un gradiente en las extremidades de los pollitos.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- La señalización celular de las células.
Sus antecedentes:
- Sonic hedgehog (Shh) es una proteína secretada vital para el desarrollo embrionario, que media el patrón a través de la señalización de corto y largo alcance.
- Los modelos de señalización Shh de largo alcance incluyen difusión simple, relé de señal y transporte mediado por citonema.
- La evidencia anterior apoyaba principalmente la difusión utilizando ShhN soluble, con datos limitados sobre Shh. nativo y libremente difusible.
Objetivo del estudio:
- Investigar la existencia y las características de una forma nativa libremente difusible de Sonic hedgehog (Shh).
- Determinar los mecanismos regulatorios que controlan la disponibilidad de esta forma de Shh difusible.
- Evaluar la relevancia fisiológica de la forma Shh libremente difusible en el patrón embrionario.
Principales métodos:
- Caracterización bioquímica de una nueva forma Shh de libre difusión (s-ShhNp).
- Evaluación de la potencia biológica y el estado multimérico de s-ShhNp.
- Análisis de la regulación por los antagonistas de la vía Shh Patched (Ptc) y la proteína que interactúa con el erizo (Hip).
- Detección de gradientes de s-ShhNp en el desarrollo de las extremidades de los pollitos.
Principales resultados:
- La evidencia presentada para una forma libremente difusible modificada por el colesterol, multimérica y biológicamente potente de Shh (s-ShhNp).
- Se ha demostrado que la disponibilidad de s-ShhNp está regulada por los antagonistas Ptc y Hip.
- Se observó un gradiente de s-ShhNp a lo largo del eje anterior-posterior de la extremidad del pollo.
Conclusiones:
- Se ha identificado una nueva forma de Sonic hedgehog (s-ShhNp), de libre difusión y biológicamente activa.
- La disponibilidad de s-ShhNp está regulada activamente por antagonistas clave en la vía Shh.
- La presencia de un gradiente s-ShhNp confirma su papel fisiológico en el patrón embrionario, particularmente en el desarrollo de las extremidades.
Videos de Conceptos Relacionados
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:23Overview of Cell Signaling
Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
01:32Types of Signaling Molecules
In multicellular organisms, many molecules transmit signals between cells to pass information. These signals vary in complexity and include small peptides, nucleotides, steroids, fatty acid derivatives, and dissolved gases such as nitric oxide. Some signaling molecules diffuse through the plasma membrane to act locally between neighboring cells or travel long distances. Others remain attached to the cell surface, transmitting information to other cells only when they make contact. In some...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:32Types of Signaling Molecules
In multicellular organisms, many molecules transmit signals between cells to pass information. These signals vary in complexity and include small peptides, nucleotides, steroids, fatty acid derivatives, and dissolved gases such as nitric oxide. Some signaling molecules diffuse through the plasma membrane to act locally between neighboring cells or travel long distances. Others remain attached to the cell surface, transmitting information to other cells only when they make contact. In some...