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Rab23 es un regulador negativo esencial de la vía de señalización Sonic hedgehog del ratón
J T Eggenschwiler1, E Espinoza, K V Anderson
1Molecular Biology Program, Sloan-Kettering Institute, New York, New York 10021, USA.
Nature
|July 13, 2001
Resumen
El gen de cerebro abierto (opb) del ratón, Rab23, actúa aguas abajo de Sonic hedgehog (Shh) para regular el desarrollo de la médula espinal. Esto revela mecanismos independientes de Shh en el patrón del tubo neural.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La neurociencia es la neurociencia.
- Genética La genética.
Sus antecedentes:
- Los genes del cerebro abierto del ratón (opb) y Sonic hedgehog (Shh) juegan roles opuestos en el patrón neural de la médula espinal.
- opb es esencial para los tipos de células dorsales, mientras que Shh es crucial para los tipos de células ventrales.
Objetivo del estudio:
- Para investigar la relación entre opb y Shh en el patrón neuronal.
- Para identificar la identidad molecular y la función del gen opb.
Principales métodos:
- La clonación basada en el mapa para identificar el gen opb.
- Análisis de Shh y Shh opb ratones mutantes dobles para estudiar el patrón neural.
- Investigando el papel de Rab23 en la vía de señalización Shh.
Principales resultados:
- El gen opb codifica Rab23, una proteína de transporte de las vesículas.
- opb actúa aguas abajo de Shh en el desarrollo de la médula espinal.
- Los tipos de células ventrales, incluida la placa de piso, están presentes en los mutantes dobles Shh opb, lo que indica mecanismos de patronaje independientes de Shh.
- Las señales de dorsalización activan la transcripción de Rab23 para inhibir la vía Shh en las células neuronales dorsales.
Conclusiones:
- Rab23 (opb) funciona río abajo de Shh para regular los destinos de las células neuronales ventrales.
- Los mecanismos independientes de Shh contribuyen al patrón dorsal-ventral del tubo neural.
- La vía Shh está regulada por señales dorsalizantes a través de Rab23.3.
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