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Cooperatividad de largo alcance en el reconocimiento molecular de ARN por oligodeoxinucleótidos con múltiples

T W Barnes¹, D H Turner

¹Department of Chemistry, University of Rochester, Rochester, New York 14627-0216, USA.

Journal of the American Chemical Society

|July 18, 2001

Resumen

Los grupos propinilo en las pirimidinas modificadas mejoran la unión y la especificidad de los oligonucleótidos. Estas modificaciones, particularmente en los dúplex de PODN:RNA, muestran una cooperación a largo plazo, ayudando a diseñar tecnologías de reconocimiento basadas en ácido nucleico.

Área de la Ciencia:

Química de oligonucleótidos y reconocimiento molecular.
La tecnología antisense y la terapéutica del ácido nucleico.

Sus antecedentes:

Las pirimidinas C5-(1-propinilo) (Y(p's) forman potentes agentes antisentido.
Comprender el papel de los grupos propinilo en el reconocimiento molecular es crucial para diseñar tecnologías efectivas basadas en ácido nucleico.

Objetivo del estudio:

Para caracterizar las contribuciones termodinámicas de los grupos propinilo en ADN: duplexos de ARN.
Para investigar el impacto de las sustituciones de grupos propinilo en la estabilidad de duplex y la geometría de hélice.
Desarrollar un modelo para predecir la estabilidad de los dúplex híbridos que contienen Y(p).

Principales métodos:

Se utilizaron experimentos de fusión UV para medir los incrementos termodinámicos asociados con las sustituciones de nucleótidos.
Se empleó espectroscopia de dicroísmo circular (CD) para analizar la geometría de la hélice.
Análisis termodinámico de duplexos de ADN:ARN modificados y no modificados con sustituciones variables.

Principales resultados:

Los grupos propinilo contribuyen significativamente a la estabilidad del dúplex, con incrementos de energía libre que van desde 0 a -4,0 kcal/mol.
El efecto estabilizador de un rA 5' no emparejado es mayor en los dúplex propinilados (PODN:RNA) que en los dúplex ADN:ARN.
La eliminación de un enlace de hidrógeno mediante la sustitución de la guanosina por la inosina tuvo un mayor efecto desestabilizador en los dúplex propinilados, que podría compensarse mediante la eliminación de un grupo propinilo.

Conclusiones:

Los grupos de propinilo exhiben una cooperatividad de largo alcance dentro de los oligonucleótidos, lo que influye en la geometría y la estabilidad de la hélice.
Estos hallazgos proporcionan información para la programación racional de oligonucleótidos con mayor unión y especificidad.
El estudio apoya el desarrollo de tecnologías de reconocimiento molecular basadas en ácido nucleico.

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