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Updated: Jun 29, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)
08:14

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

Published on: April 20, 2015

Exploradores polares: proteínas de membrana que determinan la ubicación del sitio de división

L I Rothfield1, Y L Shih, G King

  • 1Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06030, USA. lroth@neuron.uchc.edu

Cell
|July 20, 2001
PubMed
Resumen

No abstract available in PubMed .

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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...