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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
Published on: April 2, 2013
El receptor de ácido fólico alfa es un cofactor para la entrada celular de los virus Marburg y Ebola
S Y Chan1, C J Empig, F J Welte
1Gladstone Institute of Virology and Immunology, San Francisco, CA 94141, USA.
Cell
|July 20, 2001
Resumen
El receptor de ácido fólico alfa (FR-alfa) actúa como un cofactor clave para la entrada celular de los virus Marburg y Ebola. El bloqueo de FR-alfa inhibe significativamente la infección por estos letales virus de la fiebre hemorrágica.
Área de la Ciencia:
- Virología Virología.
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El virus de Marburg (MBG) y el virus del Ébola (EBO) causan fiebres hemorrágicas graves, a menudo fatales, en los seres humanos.
- La identificación de los factores de entrada celular es crucial para comprender y combatir estas infecciones virales.
Objetivo del estudio:
- Para identificar los factores celulares esenciales para la entrada del virus de Marburg.
- Investigar el papel de los factores identificados en la entrada del virus del Ébola.
Principales métodos:
- Utilizó una biblioteca de expresión retroviral y virus pseudotipados para detectar factores de entrada de MBG.
- Empleó reactivos bloqueadores del receptor de ácido fólico alfa (FR-alfa) para evaluar su papel en la entrada viral.
- Investigó la unión de FR-alfa a las glicoproteínas virales y su papel en la formación de la sincitía.
Principales resultados:
- Recuperado de ADNc que codifica el receptor de folato alfa (FR-alfa) como un factor de entrada celular para el virus MBG.
- El FR-alfa confirmado facilita la entrada tanto para los virus MBG como para los EBO.
- Se demostró que el FR-alfa se une a las glicoproteínas MBG y EBO y media la formación de sincitía.
Conclusiones:
- El receptor de ácido fólico alfa (FR-alfa) es un cofactor significativo para la entrada celular de los virus Marburg y Ebola.
- Dirigirse a FR-alfa presenta una estrategia terapéutica potencial contra las infecciones por filovirus.
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