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Videos de Conceptos Relacionados

Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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Video Experimental Relacionado

Updated: Jun 21, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

ARN: guiando el silenciamiento de genes.

M Matzke1, A J Matzke, J M Kooter

  • 1Institute of Molecular Biology, Austrian Academy of Sciences, A-5020 Salzburg, Austria. mmatzke@imb.oeaw.ac.at

Science (New York, N.Y.)
|August 11, 2001
PubMed
Resumen

Pequeños ARN de ARN de doble cadena desencadenan el silenciamiento de genes en diversos organismos. Este mecanismo de silenciamiento del ARN afecta la expresión génica post-transcripcionalmente y epigenéticamente en plantas y animales.

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • La epigenética es la epigenética.

Sus antecedentes:

  • Los ARN pequeños son reguladores clave de la expresión génica.
  • El silenciamiento del ARN es un proceso biológico conservado en diversos organismos.
  • El ARN de doble cadena (dsRNA) es un precursor para la generación de ARN pequeño.

Objetivo del estudio:

  • Para dilucidar los mecanismos de silenciamiento de genes mediados por ARN pequeño.
  • Para resaltar el papel del silenciamiento del ARN en la regulación epigenética.
  • Explorar la importancia del silenciamiento del ARN en el desarrollo.

Principales métodos:

  • Análisis de la biogénesis del ARN pequeño a partir de la escisión del ARN ds.
  • Investigando las vías de silenciamiento de genes citoplasmáticos y genómicos.

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  • Estudiar el silenciamiento de genes post-transcripcional a través de la degradación del ARNm.
  • Examinando el silenciamiento de genes transcripcionales a través de la metilación del ADN en las plantas.

    • Principales resultados:

    • Los ARN pequeños inducen el silenciamiento de genes epigenéticos en el citoplasma y en el genoma.
    • Los ARN pequeños guían la degradación de los ARN mensajeros complementarios.
    • Las plantas utilizan el silenciamiento del ARN para el silenciamiento de genes transcripcionales a través de la metilación del ADN.

    Conclusiones:

    • El silenciamiento del ARN es una potente defensa contra las secuencias extrañas.
    • Las pequeñas vías de ARN juegan un papel crucial en el desarrollo de plantas y animales.
    • Las modificaciones epigenéticas son fundamentales para la regulación genética mediada por ARN.