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Se requiere el complejo Partner of Inscuteable/Discs-large para establecer la polaridad plana durante la división
Y Bellaïche1, A Radovic, D F Woods
1Ecole Normale Supérieure, UMR 8544, 46 rue d'Ulm, 75230 Paris Cedex 05, France.
Cell
|August 18, 2001
Resumen
La señalización frizzled establece la polaridad celular al dirigir la localización de proteínas en las células precursoras de los órganos sensoriales de Drosophila. El complejo Disc-large/Partner de Inscuteable es clave para esta asimetría plana.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- La señalización Frizzled (Fz) es crucial para regular la polaridad celular entre especies.
- En Drosophila, Fz controla la división asimétrica de las células precursoras del órgano sensorial (pI) a lo largo del eje antero-posterior.
Objetivo del estudio:
- Aclarar los mecanismos moleculares por los cuales la señalización Frizzled establece la polaridad celular plana en Drosophila.
- Investigar las funciones de Discs-large (Dlg), Partner of Inscuteable (Pins) y Bazooka (Baz) en este proceso.
Principales métodos:
- Análisis de la localización de proteínas e interacciones en las células precursoras de órganos sensoriales de Drosophila.
- Manipulación genética para evaluar el requisito de Dlg, Pins y Baz para la división celular asimétrica y la localización de proteínas.
Principales resultados:
- Dlg y Pins se acumulan en la corteza anterior, mientras que Baz se localiza en la corteza posterior de las células pI.
- Dlg interactúa directamente con Pins, regulando la localización de Pins y Baz.
- Pins, junto con Fz, dirige la localización posterior de Baz, mientras que Baz no es esencial para la localización anterior de Pins.
- Tanto Baz como el complejo Dlg/Pins son necesarios para la localización asimétrica de Numb.
Conclusiones:
- El complejo Dlg/Pins actúa aguas abajo de la señalización Fz para establecer una asimetría plana en las células de Drosophila pI.
- Este estudio revela una vía molecular detallada para el establecimiento de la polaridad de las células planas que involucra las interacciones y la localización de proteínas clave.
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