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Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
Published on: July 24, 2014
La señalización FGF controla la posición de los límites de las somitas y regula el control del reloj de segmentación
J Dubrulle1, M J McGrew, O Pourquié
1Laboratoire de génétique et de physiologie du développement (LGPD), Developmental Biology Institute of Marseille (IBDM), CNRS-INSERM-Université de la méditerranée-AP de Marseille, Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France.
Cell
|August 21, 2001
Resumen
El factor de crecimiento de fibroblastos 8 (FGF8) actúa como un frente de onda en el desarrollo de los vertebrados, determinando los límites de los segmentos y la identidad axial. FGF8 asegura que la expresión del gen Hox se alinea con el número somita, no con la posición absoluta, coordinando la segmentación.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología molecular La biología molecular.
- Embriología Embriología.
Sus antecedentes:
- La segmentación de los vertebrados se basa en el reloj de segmentación en las células del mesodermo presomítico (PSM).
- Las señales precisas que posicionan los límites segmentales no se comprenden completamente.
Objetivo del estudio:
- Para investigar el papel de FGF8 en la segmentación de los vertebrados.
- Comprender cómo se determinan la posición del límite del segmento y la identidad axial.
Principales métodos:
- Estudió la expresión de FGF8 en el PSM posterior.
- Manipulación de la posición del límite en embriones de pollo.
Principales resultados:
- FGF8 forma un frente de onda en movimiento donde se determina la posición del límite del segmento y la identidad axial.
- Se encontró que la expresión del gen Hox se mantiene en el número de somitas correcto, independientemente de la posición axial absoluta.
Conclusiones:
- El FGF8 juega un papel crucial en la coordinación de la segmentación de los vertebrados.
- FGF8 asegura la activación espacio-temporal precisa de los genes Hox en relación con la formación de somitas.
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