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Un factor de "reiniciación" viral de la planta interactúa con la maquinaria de traducción del huésped
H S Park1, A Himmelbach, K S Browning
1Friedrich Miescher-Institute, P.O. Box 2543, CH-4002, Basel, Switzerland.
Cell
|September 27, 2001
Resumen
El transactivador del virus del mosaico de la coliflor (TAV) permite la reiniciación de la traducción en el ARN policistrónico. El TAV interactúa con el factor de iniciación eucariota eIF3 y las subunidades ribosómicas, facilitando la traducción de los ARNm virales.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Virología.
- Ciencias de las plantas Ciencias de las plantas.
Sus antecedentes:
- La reiniciación de la traducción es crucial para la expresión génica de los ARNm policistrónicos.
- Se sabe que el transactivador del virus del mosaico de la coliflor (TAV) controla este proceso.
- Comprender el mecanismo de TAV es clave para descifrar las estrategias de expresión génica viral.
Objetivo del estudio:
- Para aclarar el mecanismo molecular por el cual el TAV facilita la reiniciación de la traducción.
- Para identificar los factores de acogida involucrados en la traducción mediada por TAV.
- Para caracterizar la interacción de TAV con la maquinaria de traducción del anfitrión.
Principales métodos:
- Ensayos bioquímicos in vitro e in vivo para estudiar las interacciones de las proteínas.
- Estudios de asociación de los polisomas.
- Expresión transitoria en los protoplastos de las plantas.
- Análisis de la formación del complejo ternario.
Principales resultados:
- TAV se asocia con los polisomas y el factor de iniciación eucariota eIF3.3.
- TAV interactúa físicamente con la subunidad eIF3 eIF3g y la proteína ribosómica 60S L24.
- La expresión de eIF3g y L24 impacta la reiniciación mediada por TAV.
- Se demostró la formación de complejos ternales TAV/eIF3/40S y eIF3/TAV/60S.
Conclusiones:
- TAV recluta eIF3 a los polisomas, lo que permite la reiniciación de la traducción en el ARN policistrónico.
- Este mecanismo permite que el virus supere las barreras celulares a la traducción.
- TAV utiliza los factores huésped eIF3g y L24 para lograr una eficiente expresión génica viral.
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