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Una extensa clase de pequeños ARN en Caenorhabditis elegans

R C Lee¹, V Ambros

¹Dartmouth Medical School, Department of Genetics, Hanover, NH 03755, USA.

Science (New York, N.Y.)

|October 27, 2001

Resumen

Los investigadores descubrieron 15 nuevos genes de microARN en C. elegans, revelando que estos pequeños ARN son antiguos y diversos reguladores del desarrollo. Algunos microARN de C. elegans tienen contrapartes en mamíferos e insectos.

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología del desarrollo Biología del desarrollo.
Genética La genética.

Sus antecedentes:

Los ARN antisense como lin-4 y let-7 regulan el tiempo de desarrollo en C. elegans inhibiendo la traducción del ARNm.
La conservación del ARN let-7 en animales bilaterales sugiere que los microARN (miRNA) son evolutivamente antiguos.

Objetivo del estudio:

Para identificar nuevos genes de microARN en C. elegans.
Investigar la diversidad y la expresión del desarrollo de microARN en C. elegans.

Principales métodos:

Análisis bioinformático para predecir los genes de miRNA.
clonación de ADNc para confirmar los genes de miARN identificados.
Análisis de la abundancia de transcripción de ARN pequeño durante el desarrollo larval de C. elegans.

Principales resultados:

Se identificaron quince nuevos genes de miRNA en C. elegans.
Varios miRNAs identificados exhibieron diferentes niveles de transcripción durante el desarrollo larval.
Se encontraron homólogos de tres miRNA de C. elegans en mamíferos y/o insectos.

Conclusiones:

La clase de microARN de pequeños ARN no codificantes es abundante y diversa en C. elegans.
La conservación evolutiva de algunos miRNAs destaca sus orígenes antiguos y roles fundamentales en el desarrollo.

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