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Interrupciones dinámicas en la arquitectura y la integridad de la envoltura nuclear inducidas por el VIH-1 Vpr
C M de Noronha1, M P Sherman, H W Lin
1Gladstone Institute of Virology and Immunology, Department of Medicine, University of California, San Francisco, CA 94103, USA.
Science (New York, N.Y.)
|November 3, 2001
Resumen
El virus de la inmunodeficiencia humana-1 (VIH-1) La proteína Vpr causa la detención del ciclo celular al inducir hernias de la envoltura nuclear. Estas rupturas mezclan componentes nucleares y citoplasmáticos, lo que probablemente contribuye a la detención del punto de control G2.
Área de la Ciencia:
- Biología celular Biología celular.
- Virología Virología.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Se sabe que la proteína Vpr del virus de la inmunodeficiencia humana-1 (VIH-1) detiene la proliferación celular en la fase G2.
- La progresión del ciclo celular, en particular la transición G2 a la mitosis, se basa en la fosforilación regulada y la compartimentación de proteínas clave.
Objetivo del estudio:
- Investigar el tráfico intracelular de reguladores del ciclo celular durante la detención del ciclo celular inducida por el Vpr del VIH-1.
- Para aclarar el mecanismo por el cual Vpr induce la detención de G2.
Principales métodos:
- Estudió el tráfico intracelular de reguladores del ciclo celular en células humanas que expresan el tipo salvaje y el VIH-1 Vpr. mutante.
- Morfología observada de la envoltura nuclear (NE) y integridad de la lámina nuclear.
Principales resultados:
- El Vpr de tipo salvaje, a diferencia de los mutantes con deficiencia de detención de G2, indujo hernias transitorias y localizadas en la envoltura nuclear.
- Estas hernias de NE inducidas por Vpr estaban relacionadas con defectos en la lámina nuclear.
- Se observó la ruptura intermitente de las hernias de NE, lo que lleva a la mezcla de contenidos nucleares y citoplasmáticos.
Conclusiones:
- El VIH-1 Vpr induce alteraciones significativas en la estructura de la envoltura nuclear.
- Las hernias y rupturas de la envoltura nuclear mediadas por Vpr son mecanismos probables que contribuyen a la detención del ciclo celular G2.
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