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Inducción de la diferenciación de las células dendríticas por IFN-alfa en el lupus eritematoso sistémico
P Blanco1, A K Palucka, M Gill
1Baylor Institute for Immunology Research, Dallas, TX 75204, USA.
Science (New York, N.Y.)
|November 17, 2001
Resumen
El lupus eritematoso sistémico (LES) puede deberse a una alteración de la función de las células dendríticas (CE). En pacientes con LES, el suero impulsa a los monocitos normales a convertirse en DC, lo que potencialmente alimenta las respuestas autoinmunes a través del interferón alfa (IFN-alfa).
Área de la Ciencia:
- Inmunología Inmunología.
- La autoinmunidad es autoinmunidad.
- Biología celular Biología celular.
Sus antecedentes:
- Las células dendríticas (CD) son reguladores cruciales de las respuestas inmunes y la tolerancia.
- El lupus eritematoso sistémico (LES) es una enfermedad autoinmune que involucra linfocitos autorreactivos.
- Se supone que las alteraciones en la función DC contribuyen a la patogénesis del LES.
Objetivo del estudio:
- Para investigar el papel de las células dendríticas (CD) en el lupus eritematoso sistémico (LES).
- Para examinar la función de los monocitos y DC derivados de la sangre de pacientes con LES.
- Para determinar la influencia del suero de pacientes con LES en la diferenciación de monocitos en DCs.
Principales métodos:
- Análisis in vitro de la función de los monocitos de pacientes con LES.
- Inducción de la diferenciación de las células dendríticas (CD) de los monocitos normales utilizando suero de pacientes con LES.
- Evaluación de las capacidades de captura y presentación de antígenos DC.
- Análisis de correlación entre la capacidad de diferenciación de DC, la actividad de la enfermedad y los niveles de interferón alfa (IFN-alfa).
Principales resultados:
- Los monocitos de pacientes con LES exhibieron actividad celular presentadora de antígenos in vitro.
- El suero de pacientes con LES indujo a los monocitos normales a diferenciarse en DC funcionales.
- Estos DC capturaron antígenos de las células moribundas y los presentaron a las células T CD4-positivas.
- La capacidad del suero de LES para inducir la diferenciación de DC se correlacionó con la actividad de la enfermedad de LES y fue dependiente del interferón alfa (IFN-alfa).
Conclusiones:
- La alteración de la función de las células dendríticas (CD), particularmente su inducción por el interferón alfa (IFN-alfa), puede ser un factor clave en la patogénesis del lupus eritematoso sistémico (LES).
- El suero de los pacientes con LES promueve la diferenciación de los monocitos en DC, que pueden presentar autoantígenos.
- Este proceso, impulsado por IFN-alfa, puede contribuir a la respuesta autoinmune en el LES.
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