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La inhibición de la activación de JNK a través de los genes diana NF-kappaB
G Tang1, Y Minemoto, B Dibling
1Ben May Institute for Cancer Research, Committee on Cancer Biology, University of Chicago, 5841 S. Maryland Avenue, MC 6027, Chicago, Illinois 60637, USA.
Nature
|November 20, 2001
Resumen
La señalización del factor de necrosis tumoral alfa (TNF-alfa) implica interacciones complejas. La vía IKK/NF-kappaB inhibe la apoptosis inducida por TNF-alfa al suprimir la activación de JNK, en parte a través de XIAP.
Área de la Ciencia:
- Biología Celular y Molecular.
- Inmunología Inmunología.
- Transducción de señales Transducción de señales.
Sus antecedentes:
- El factor de necrosis tumoral alfa (TNF-alfa) es un regulador clave de las respuestas inmunes, la inflamación y la apoptosis.
- La determinación del destino celular tras la exposición al TNF-alfa se basa en la integración de señales de efectores como la IkappaB quinasa (IKK), la c-Jun N-terminal proteína quinasa (JNK) y las caspasas.
- La interacción entre las vías IKK/NF-kappaB y JNK en la apoptosis inducida por TNF-alfa sigue siendo incompletamente entendida.
Objetivo del estudio:
- Investigar el cruce entre las vías de señalización IKK y JNK en respuesta al TNF-alfa.
- Para aclarar el papel de este cruce en la modulación de la apoptosis inducida por el TNF-alfa.
Principales métodos:
- Se utilizaron fibroblastos embrionarios murinos con deficiencia de IKKbeta o RelA/p65 (subunidad de NF-kappaB).
- Se analizó el impacto de la modulación de la vía IKK/NF-kappaB en la activación del JNK mediada por TNF-alfa.
- Se evaluó la contribución del inhibidor de la apoptosis vinculado al cromosoma X (XIAP) en este cruce.
Principales resultados:
- La vía IKK/NF-kappaB modula negativamente la activación del JNK mediada por el TNF-alfa.
- Este cruce negativo está mediado en parte por el XIAP inducido por NF-kappaB.
- La modulación negativa observada de la activación de la JNK es específica para la señalización del TNF-alfa y no afecta la activación de la JNK por IL-1.
Conclusiones:
- La vía IKK/NF-kappaB suprime activamente la activación del JNK aguas abajo del TNF-alfa.
- Este cruce inhibidor, que involucra a XIAP, contribuye a la inhibición general de la apoptosis inducida por el TNF-alfa.
- Los hallazgos revelan un nuevo mecanismo de regulación cruzada entre las principales vías de señalización inflamatoria.
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