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05:12Fine Adjustment of Caenorhabditis elegans Orientation on Channeled Agar Pads for Imaging Neuroregeneration
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Here, we present a protocol for fabricating channeled agar pads using PDMS molds created from vinyl records. The channels enable users to finely orient Caenorhabditis elegans to improve imaging contrast and facilitate the comparison of structures. These capabilities are particularly useful in neuroregeneration studies.
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05:46Correction of Presbyopia by Monocular Bi-Aspheric Ablation Profile
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This article provides a step-by-step guide to correcting presbyopia with a monocular bi-aspheric ablation...
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08:12An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
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This protocol is a beginner's entryway into processing, fitting, and interpreting transient absorption spectra. The focus of this protocol is the preparation of datasets, and fitting using both single wavelength kinetics and global lifetime analysis. Challenges associated with transient absorption data and its fitting are discussed.
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08:18High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
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Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software,...
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10:09Operation of the Collaborative Composite Manufacturing (CCM) System
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A collaborative composite manufacturing system is developed for robotic lay-up of composite laminates using the prepreg tape. The proposed system allows the production of composite laminates with high levels of geometrical complexity. The issues in the path planning, coordination of the robots and control are addressed in the proposed...
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11:08Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
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A non-labeled, non-radio-isotopic method for DNA polymerase proofreading and a DNA repair assay was developed by using high-resolution MALDI-TOF mass spectrometry and a single nucleotide extension strategy. The assay proved to be very specific, simple, rapid, and easy to perform for proofreading and repair patches shorter than 9-nucleotides.
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