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pre-mRNA Processing

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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Published on: September 29, 2012

Biología celular. biología celular. El aprovechamiento para la exportación de ARNm.

M J Moore¹, M Rosbash

¹Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA. mmoore@brandeis.edu

Science (New York, N.Y.)

|December 1, 2001

Resumen

Este resumen es generado por máquina.

Los investigadores desarrollaron un método novedoso utilizando inhibidores de péptidos para rastrear las vías de exportación de ARN mensajero (ARNm) desde el núcleo hasta el citoplasma en las células vivas.

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Área de la Ciencia:

Biología Molecular Biología Molecular
Biología celular Biología celular.
Genética La genética.

Sus antecedentes:

La exportación de ARN mensajero (ARNm) desde el núcleo al citoplasma es crucial para la expresión génica.
La identificación de vías de exportación específicas para ARNm individuales ha sido un desafío significativo en la biología molecular.

Objetivo del estudio:

Presentar una nueva estrategia para investigar las rutas de exportación de ARNm.
Para permitir el estudio de los mecanismos de transporte de ARNm en las células vivas.

Principales métodos:

Discusión de un nuevo enfoque que utiliza inhibidores de péptidos que penetran en las células.
Aplicación de estos inhibidores para desentrañar vías específicas de exportación de ARNm.

Principales resultados:

La estrategia propuesta ofrece una nueva forma de diseccionar complejas rutas de exportación de ARNm.
Permite el análisis en tiempo real de la dinámica de transporte de ARNm dentro de las células vivas.

Conclusiones:

Este método innovador proporciona una poderosa herramienta para comprender la exportación nuclear de ARNm.
Facilita una investigación detallada sobre las funciones de los adaptadores y receptores de exportación.