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La matanza rápida de Streptococcus pneumoniae con una hidrolasa de la pared celular de los bacteriófagos
J M Loeffler1, D Nelson, V A Fischetti
1Laboratory of Bacterial Pathogenesis, The Rockefeller University, New York, NY 10021, USA.
Science (New York, N.Y.)
|December 12, 2001
Resumen
Una nueva enzima, Pal, elimina rápidamente Streptococcus pneumoniae, incluidas las cepas resistentes, de la nasofaringe. Esta enzima muestra una alta especificidad y previene la resistencia, ofreciendo una nueva intervención potencial para el transporte de neumocócicos.
Área de la Ciencia:
- Microbiología Microbiología.
- Biotecnología La biotecnología es la biotecnología.
- Enfermedades infecciosas Enfermedades infecciosas.
Sus antecedentes:
- El transporte nasofaríngeo de Streptococcus pneumoniae es un reservorio significativo para la transmisión comunitaria.
- Las intervenciones actuales para eliminar el transporte de neumocócicos son limitadas, especialmente para las cepas resistentes a los antibióticos.
Objetivo del estudio:
- Evaluar la eficacia de una enzima lítica purificada de bacteriófago neumocócico (Pal) en la eliminación rápida de Streptococcus pneumoniae.
- Para evaluar la especificidad de la enzima y su potencial para el desarrollo de resistencia.
Principales métodos:
- Pruebas in vitro de la enzima Pal contra 15 serotipos comunes de Streptococcus pneumoniae, incluidas las cepas resistentes a la penicilina.
- Estudios in vivo utilizando un modelo de ratón de colonización neumocócica para evaluar la eficacia de la enzima y el impacto en los títulos bacterianos.
- Evaluación del efecto de la enzima Pal en otros microorganismos orofaríngeos y evaluación de los neumococos resistentes a Pal.
Principales resultados:
- La enzima Pal demostró la matanza rápida de 15 serotipos comunes de Streptococcus pneumoniae en cuestión de segundos después del contacto.
- In vivo, un solo tratamiento con la enzima Pal produjo en ratones previamente colonizados títulos neumocócicos indetectables después de 5 horas.
- La enzima mostró un impacto mínimo o nulo en otros microbios orofaríngeos, y no se detectaron neumococos resistentes a Pal después de una exposición extensa.
Conclusiones:
- La enzima Purified Pal es un agente altamente eficaz y rápido para la eliminación de Streptococcus pneumoniae.
- La enzima Pal exhibe una excelente especificidad, apuntando a los neumococos sin dañar la flora comensal y sin inducir resistencia.
- Pal representa un prometedor nuevo candidato terapéutico para el control de la enfermedad neumocócica al atacar su reservorio.
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