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El factor de acoplamiento mitocondrial 6 está presente en la superficie de las células endoteliales vasculares
1Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan. osanait@cc.hirosaki-u.ac.jp
Circulation
|December 19, 2001
Resumen
Las células endoteliales vasculares liberan el factor de acoplamiento mitocondrial 6 (CF6), una sustancia presora. El estrés de cizallamiento aumenta significativamente la liberación de CF6 de estas células, lo que indica su regulación en la circulación.
Área de la Ciencia:
- Biología Cardiovascular Biología Cardiovascular
- Fisiología Celular Fisiología celular
- La bioquímica es la bioquímica.
Sus antecedentes:
- El factor de acoplamiento mitocondrial 6 (CF6) es un inhibidor endógeno de la síntesis de prostaciclina.
- CF6 circula en la circulación sistémica y actúa como una sustancia presora en ratas.
- Se investigó el origen del CF6 circulante, centrándose en las células endoteliales vasculares.
Objetivo del estudio:
- Para determinar si las células endoteliales vasculares son una fuente de CF6 circulante.
- Para investigar el efecto de la tensión de cizallamiento de fluidos en la liberación de CF6 de las células endoteliales.
Principales métodos:
- Se utilizaron células endotelial de la vena umbilical humana (HUVEC) y células ECV 304 cultivadas.
- Microscopía de inmunofluorescencia para detectar CF6 asociado a la superficie.
- Concentración de CF6 medida en el medio de cultivo bajo condiciones de estrés estático y de corte.
- Se analizó el contenido de CF6 en los homogenatos celulares y las mitocondrias.
- Se ha evaluado la relación entre el ARNm CF6 y GAPDH.
- Citometría de flujo para cuantificar el CF6 asociado a la superficie.
Principales resultados:
- La inmunofluorescencia confirmó el CF6 asociado a la superficie tanto en ECV 304 como en HUVEC.
- La concentración de CF6 en el medio aumentó con el tiempo en condiciones estáticas.
- La tensión de cizallamiento de fluidos aumentó significativamente la liberación de CF6, con concentraciones que aumentan dramáticamente en niveles de cizallamiento más altos (25 dines / cm2).
- El estrés de cizallamiento aumentó el contenido de CF6 en los homogenatos celulares y las mitocondrias a 15 dines/cm2.
- El estrés de cizallamiento elevó la relación entre el ARNm CF6 y GAPDH.
- El CF6 asociado a la superficie aumentó después de la exposición a la tensión de cizallamiento.
Conclusiones:
- Las células endoteliales vasculares son una fuente significativa de CF6.
- La liberación de CF6 asociado a la superficie de las células endoteliales está regulada por la tensión de cizallamiento de fluidos.
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