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El nuevo factor de transcripción osterix que contiene el dedo de zinc es necesario para la diferenciación de los
Kazuhisa Nakashima1, Xin Zhou, Gary Kunkel
1Department of Molecular Genetics and Program in Genes and Development, M. D. Anderson Cancer Center, University of Texas, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
Cell
|January 17, 2002
Resumen
Osterix (Osx) es un factor de transcripción crucial para la formación ósea. Osx actúa aguas abajo de Runx2/Cbfa1, y su ausencia impide la diferenciación de los osteoblastos, deteniendo el desarrollo óseo.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- El desarrollo óseo se basa en la regulación precisa de la diferenciación de los osteoblastos.
- Los factores de transcripción juegan un papel crítico en el control de la formación esquelética.
- Los mecanismos moleculares que gobiernan la osteogénesis son complejos e incompletamente comprendidos.
Objetivo del estudio:
- Identificar nuevos factores de transcripción involucrados en la formación ósea.
- Para aclarar el papel de Osterix (Osx) en el desarrollo esquelético.
- Determinar la relación entre Osx y Runx2/Cbfa1 en la osteogénesis.
Principales métodos:
- Generación y análisis de ratones nulos Osx.
- Análisis de los ratones sin Runx2/Cbfa1.
- Análisis de la expresión génica de los tejidos esqueléticos.
Principales resultados:
- Osterix (Osx) es un nuevo factor de transcripción del dedo de zinc expresado específicamente en los huesos en desarrollo.
- Los ratones Osx null muestran una ausencia completa de formación ósea.
- Las células mesenquimales en ratones nulos Osx no logran diferenciarse en osteoblastos, a pesar de expresar Runx2/Cbfa1.1.
- La expresión de Osx depende de Runx2/Cbfa1, lo que indica que Osx actúa aguas abajo.
Conclusiones:
- Osx es esencial para la diferenciación de los osteoblastos y la formación ósea.
- Osx funciona aguas abajo de Runx2/Cbfa1 en la vía osteogénica.
- Los preosteoblastos que expresan Runx2/Cbfa1 pueden ser bipotenciales, con Osx dirigiéndolos hacia el linaje de los osteoblastos.
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