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Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
Published on: September 15, 2010
Integrin alpha3beta1 (CD 49c/29) es un receptor celular para el herpesvirus asociado al sarcoma de Kaposi
Shaw M Akula1, Naranatt P Pramod, Fu Zhang Wang
1Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, KS 66160, USA.
Cell
|February 21, 2002
Resumen
El herpesvirus humano-8 (HHV-8) utiliza la integrina alfa3beta1 para la entrada en las células, crucial para Kaposi.
Área de la Ciencia:
- Virología Virología.
- Biología celular Biología celular.
- Inmunología Inmunología.
Sus antecedentes:
- El herpesvirus humano-8 (HHV-8) está relacionado con el desarrollo del sarcoma de Kaposi.
- La glicoproteína B del sobre HHV-8 contiene un motivo RGD involucrado en las interacciones celulares.
Objetivo del estudio:
- Para investigar el papel de las integrinas en la infección por HHV-8.
- Para identificar moléculas de integrina específicas que median la entrada del HHV-8.
Principales métodos:
- Pruebas de inhibición utilizando péptidos RGD y anticuerpos anti-integrina.
- Evaluación de la infectividad en células con expresión alterada de alfa-3 integrina.
- Inmunoprecipitación de los complejos virus-integrina.
- Análisis de la activación de la cinasa de adhesión focal (FAK).
Principales resultados:
- La infectividad del HHV-8 fue reducida por los péptidos RGD y los anticuerpos dirigidos a las integrinas alfa3beta1.
- El aumento de la expresión de alfa3 integrina aumentó la infectividad del HHV-8.
- Los estudios de unión al virus indicaron el papel de la integrina alfa3beta1 en la entrada del HHV-8.
- La infección por HHV-8 activó FAK, lo que sugiere una señalización mediada por integrina.
Conclusiones:
- La integrina alfa3beta1 es esencial para la entrada del HHV-8 en las células diana.
- Las vías de señalización asociadas a la integrina están involucradas en la infección por HHV-8.
- La orientación de la integrina alfa3beta1 puede ofrecer estrategias terapéuticas contra las enfermedades asociadas al HHV-8.
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