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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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La cooperación entre los complejos que regulan la estructura de la cromatina y la transcripción.

Geeta J Narlikar¹, Hua-Ying Fan, Robert E Kingston

¹Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

|March 23, 2002

Resumen

Los complejos de remodelación de la cromatina y las enzimas HAT/HDAC se coordinan para superar las barreras de transcripción. Esto asegura la accesibilidad del ADN para la maquinaria de transcripción en los eucariotas.

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Área de la Ciencia:

Biología Molecular Biología Molecular
La epigenética es la epigenética.
Regulación genética Reglamento genético.

Sus antecedentes:

La estructura de la cromatina presenta importantes barreras físicas para la transcripción eucariota.
El acceso a la plantilla de ADN es un punto de regulación crítico para la expresión génica.

Objetivo del estudio:

Discutir las actividades coordinadas de los complejos modificadores de cromatina en la regulación de la transcripción.
Aclarar los mecanismos para crear una plantilla de ADN accesible para la transcripción.

Principales métodos:

Revisión de la literatura existente sobre los modificadores de la cromatina.
Análisis de la interacción entre los complejos de remodelación dependientes de ATP y los complejos HAT/HDAC.

Principales resultados:

Dos clases principales de modificadores de la cromatina, los complejos de remodelación dependientes de ATP y los complejos HAT / HDAC, son jugadores clave.
La coordinación entre estos complejos es esencial para superar las barreras de la cromatina.

Conclusiones:

La acción coordinada de los remodeladores dependientes de ATP y los HAT/HDAC facilita la formación de una plantilla de ADN abierta.
Este proceso es crucial para permitir que el aparato general de transcripción inicie la transcripción en los eucariotas.