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Updated: Jul 17, 2026
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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
Papel de una modificación similar a la ubiquitina en la morfogénesis polarizada
Gunnar A G Dittmar1, Caroline R M Wilkinson, Paul T Jedrzejewski
1Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Science (New York, N.Y.)
|March 30, 2002
Resumen
Los investigadores identificaron Hub1, un modificador de proteínas en la levadura, crucial para la polaridad celular y el apareamiento. Su ausencia interrumpe la localización de proteínas y perjudica las funciones reproductivas.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- Las proteínas tipo I similares a la ubiquitina son modificadores críticos de las proteínas.
- La comprensión de nuevos modificadores como Hub1 es esencial para elucidar los procesos celulares.
Objetivo del estudio:
- Identificar y caracterizar un nuevo modificador de proteína post-traducional, Hub1, en Saccharomyces cerevisiae.
- Para investigar el papel de Hub1 en los procesos celulares, incluyendo la conjugación de proteínas, la localización y el apareamiento.
Principales métodos:
- Identificación y caracterización de las proteínas en Saccharomyces cerevisiae.
- Estudios de sobreexpresión para analizar la formación conjugada.
- Análisis de fenotipos mutantes (hub1Delta) incluyendo localización subcelular y ensayos de apareamiento.
Principales resultados:
- Hub1 fue identificado como un modificador de proteína post-traducional.
- Los objetivos de conjugación de Hub1 incluyen factores de polaridad celular Sph1 y Hbt1.1.
- hub1Los mutantes Delta exhiben una localización subcelular interrumpida de Sph1 y Hbt1, lo que lleva a una polarización celular defectuosa y al apareamiento.
Conclusiones:
- Hub1 es un modificador de proteínas funcional similar a la ubiquitina involucrado en la regulación de la polaridad celular y el apareamiento en la levadura.
- Se sugiere el procesamiento proteolítico para producir Hub1.1. maduro.
- La conjugación Hub1 es crítica para la localización adecuada de sus objetivos y la función celular en general.
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These groups modify specific amino acids in a protein.
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Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
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It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
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Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
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