Video Experimental Relacionado
Updated: Jun 26, 2026
08:34
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
Published on: April 13, 2015
La senescencia de las células endoteliales en la aterosclerosis humana: papel de los telómeros en la disfunción
Tohru Minamino1, Hideaki Miyauchi, Toshihiko Yoshida
1Department of Cardiovascular Science and Medicine, Chiba University Graduate School of Medicine, Chiba, Japan.
Circulation
|April 3, 2002
Resumen
La senescencia celular, caracterizada por el acortamiento de los telómeros, está presente en las lesiones ateroscleróticas humanas. Esta senescencia en las células endoteliales vasculares contribuye al desarrollo de la aterosclerosis y los trastornos vasculares relacionados.
Área de la Ciencia:
- Investigación cardiovascular Investigación cardiovascular Investigación cardiovascular Investigación cardiovascular Investigación cardiovascular
- Biología celular Biología celular.
- Investigación sobre el envejecimiento Investigación sobre el envejecimiento.
Sus antecedentes:
- La senescencia celular está relacionada con el envejecimiento y los trastornos vasculares.
- Los telómeros y la telomerasa juegan un papel en la senescencia de las células vasculares.
- El acortamiento de los telómeros in vivo sugiere un papel en la aterosclerosis.
Objetivo del estudio:
- Para investigar la presencia de células vasculares senescentes en lesiones ateroscleróticas humanas.
- Para determinar si la senescencia de las células endoteliales contribuye a la aterogénesis.
Principales métodos:
- Examinó la actividad de la beta-galactosidasa asociada a la senescencia en las arterias coronarias y mamarias internas humanas.
- Utilizó inmunohistoquímica para identificar las células positivas para la beta-galactosidasa.
- Senescencia inducida en las células endoteliales aórticas humanas (HAEC) para evaluar los cambios funcionales.
- Investigó el efecto de la introducción de la telomerasa en las HAEC senescentes.
Principales resultados:
- Se observó una fuerte tinción de beta-galactosidasa en lesiones ateroscleróticas de las arterias coronarias.
- Las células positivas para beta-galactosidasa fueron identificadas como células endoteliales vasculares.
- Las HAEC senescentes mostraron un aumento de la expresión de ICAM-1 y una disminución de la actividad eNOS.
- La introducción de telomerasa extendió la vida útil de HAEC e inhibió los cambios funcionales asociados a la senescencia.
Conclusiones:
- Las células endoteliales vasculares senescentes están presentes en las lesiones ateroscleróticas humanas.
- La senescencia de las células endoteliales, inducida por el acortamiento de los telómeros, puede contribuir a la aterogénesis.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:42The Extracellular Matrix
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:23Tissue Renewal without Stem Cells
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
01:26Extracellular Matrix
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
01:19The Effect of Aging on Tissues
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...