Video Experimental Relacionado
Updated: Jul 9, 2026
13:22
Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
Published on: November 2, 2011
Estructura del ectodominio de la C-cadherina e implicaciones para los mecanismos de adhesión celular
Titus J Boggon1, John Murray, Sophie Chappuis-Flament
1Department of Biochemistry, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA.
Science (New York, N.Y.)
|April 20, 2002
Resumen
La estructura cristalina de la C-cadherina revela cómo se unen estas proteínas de adhesión celular. Este hallazgo proporciona un nuevo marco para la comprensión de las interacciones de la cadherina en los tejidos animales.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología Estructural Biología estructural.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las cadherinas son proteínas transmembrana cruciales responsables de la adhesión célula-célula en los tejidos animales.
- Comprender la función de la cadherina es vital para comprender el desarrollo y la integridad de los tejidos.
Objetivo del estudio:
- Para determinar la estructura cristalina de alta resolución del dominio extracelular de la C-cadherina.
- Para dilucidar los mecanismos moleculares subyacentes a la adhesión celular clásica mediada por cadherina.
Principales métodos:
- Se empleó cristalografía de rayos X para obtener la estructura de resolución de 3.1 angstroms.
- El estudio se centró en todo el dominio extracelular funcional de la C-cadherina.
Principales resultados:
- La estructura cristalina del dominio extracelular de la C-cadherina fue determinada a una resolución de 3.1 angstroms.
- Se propuso un mecanismo molecular para la adhesión de la trans-caderina, que involucra un triptófano conservado.
- La estructura ofrece información sobre las interacciones de cis y trans cadherinas.
Conclusiones:
- La estructura determinada proporciona una base molecular para la clásica adhesión celular mediada por cadherina.
- Este trabajo establece un nuevo marco para estudiar las interacciones de la cadherina a nivel celular.
Videos de Conceptos Relacionados
01:25Structure of Cadherins
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
01:23Catenins
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the adherens...
01:24Adherens Junctions
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Adherens Junctions are Dynamic
The endothelial cells...
01:19Cadherins in Tissue Organization
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
01:20Cell Adhesion Molecules - Types and Functions
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved in a...
CAM Families
The Integrin family of proteins is primarily involved in a...
01:26Tension Response at Adherens Junctions
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin homology) domains...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin homology) domains...