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Un complejo con modificadores de cromatina que ocupa los genes E2F y Myc-responsivos en las células G0
Hidesato Ogawa1, Kei-Ichiro Ishiguro, Stefan Gaubatz
1Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA.
Science (New York, N.Y.)
|May 11, 2002
Resumen
Los complejos de proteínas E2F-6 silencian los genes a través de modificadores de la cromatina en las células quiescentes. Este mecanismo implica modificaciones de histonas y proteínas del grupo Polycomb, independientemente de las proteínas del retinoblastoma.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La epigenética es la epigenética.
- Regulación del ciclo celular Reglamento del ciclo celular.
Sus antecedentes:
- E2F-6 es un factor de transcripción involucrado en el silenciamiento de genes.
- Su mecanismo de represión, independiente de las proteínas del retinoblastoma, requiere una mayor aclaración.
Objetivo del estudio:
- Para purificar y caracterizar el complejo proteico E2F-6.
- Para comprender los mecanismos moleculares subyacentes al silenciamiento de genes mediado por E2F-6.
Principales métodos:
- Purificación del complejo E2F-6 a partir de células cultivadas.
- Identificación de las proteínas asociadas y modificadores de la cromatina.
- Análisis de la ocupación del promotor objetivo en diferentes fases del ciclo celular.
Principales resultados:
- E2F-6 forma un complejo multimérico con Mga y Max, que se une a los sitios E2F, Myc y Brachyury.
- El complejo contiene la nueva histona metiltransferasa (H3K9), HP1gamma y las proteínas del grupo Polycomb.
- El complejo E2F-6 se une preferentemente a los promotores objetivo en las células G0 (quiescentes) sobre las células G1.
Conclusiones:
- Los modificadores de cromatina dentro del complejo E2F-6 median el silenciamiento de genes.
- Este silenciamiento afecta a los genes receptivos a E2F y Myc en las células en reposo.
- E2F-6 juega un papel importante en la regulación génica dependiente del ciclo celular a través de mecanismos epigenéticos.
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