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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
Published on: October 28, 2014
Cid13 es una polimerasa poli (A) citoplasmática que regula la ribonucleótido reductasa mRNA
Shigeaki Saitoh1, Andrei Chabes, W Hayes McDonald
1Department of Molecular Biology, The Scripts Research Institute, La Jolla, CA 92037, USA.
Cell
|June 14, 2002
Resumen
La levadura de fisión Cid13 es una polimerasa poli (A) citoplasmática que se dirige específicamente al ARNm suc22. Esta enzima es crucial para mantener la replicación del ADN y la estabilidad del genoma mediante la regulación de las piscinas de nucleótidos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
- Genética La genética.
Sus antecedentes:
- La levadura de fisión Cid13 y la levadura en ciernes Trf4/5 pertenecen a una familia de nucleotidiltransferasas conservadas.
- Trf4/5 se consideran actualmente las polimerasas de ADN esenciales.
- La función precisa de Cid13 dentro de la célula aún no se ha aclarado completamente.
Objetivo del estudio:
- Caracterizar la actividad enzimática y la localización celular de la levadura de fisión Cid13.
- Para determinar las dianas específicas de ARNm de Cid13.
- Para investigar el papel de Cid13 en la replicación del ADN y el mantenimiento del genoma.
Principales métodos:
- Pruebas enzimáticas para determinar la actividad de poliadenilación.
- Estudios de localización celular utilizando el microscopio.
- Análisis de los mutantes cid13 para las piscinas dNTP y la sensibilidad a la hidroxiurea.
- Identificación de la diana del ARNm utilizando técnicas de biología molecular.
Principales resultados:
- Cid13 funciona como una poli (A) polimerasa, distinta de las poliadenilasas nucleares.
- Cid13 está localizado en el citoplasma.
- Cid13 específicamente poliadenila el ARNm suc22, que codifica una subunidad de la ribonucleótido reductasa (RNR).
- Los mutantes del cid13 muestran reducción de las reservas de dNTP y sensibilidad a la hidroxiurea.
Conclusiones:
- Cid13 representa una nueva polimerasa poli (A) citoplasmática.
- Cid13 juega un papel crítico en la regulación de las piscinas de nucleótidos esenciales para la replicación del ADN.
- Cid13 es importante para el mantenimiento del genoma a través de su orientación específica de la subunidad RNR del ARNm.
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