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Videos de Conceptos Relacionados

Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
Receptor-Mediated Endocytosis01:20

Receptor-Mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

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Updated: Jun 19, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 27, 2011

Los adaptadores Clathrin se adaptan muy bien.

Tom Kirchhausen1

  • 1Department of Cell Biology, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA. kirchhausen@xta1200.harvard.edu

Cell
|June 28, 2002
PubMed
Resumen
Este resumen es generado por máquina.

La vía de la clatrina media la endocitosis y la regulación a la baja del factor de crecimiento. Los investigadores determinaron la estructura atómica del núcleo de la proteína adaptadora 2 (AP-2), revelando su papel en la vinculación de las capas de clatrina a las proteínas de carga.

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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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In vivo and in vitro Studies of Adaptor-clathrin Interaction
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Área de la Ciencia:

  • Biología celular Biología celular.
  • Biología molecular y estructural Biología molecular y estructural

Sus antecedentes:

  • La vía de la clatrina es crucial para la endocitosis mediada por receptores y la regulación de la señalización del factor de crecimiento.
  • Los adaptadores de clatrina conectan la capa de clatrina a las proteínas de carga transmembrana para la clasificación en vesículas.

Objetivo del estudio:

  • Para dilucidar la estructura atómica del núcleo de la proteína adaptadora 2 (AP-2).
  • Para entender cómo AP-2 interactúa con la membrana celular y las proteínas de carga.

Principales métodos:

  • Cristalografía de rayos X o microscopía crioectrónica para determinar la estructura atómica.
  • Pruebas bioquímicas para estudiar las interacciones proteína-proteína.

Principales resultados:

  • Se ha determinado la estructura atómica del núcleo AP-2.
  • La estructura revela las interacciones específicas entre AP-2, la membrana y las colas citosólicas de la proteína de carga.

Conclusiones:

  • La estructura resuelta proporciona una visión crítica de los mecanismos moleculares de la endocitosis mediada por clatrina.
  • Comprender la estructura de AP-2 facilita la investigación sobre el tráfico de receptores y las vías de señalización celular.