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Detecting Cortex Fragments During Bacterial Spore Germination
Published on: June 25, 2016
Identificación sistemática de las vías que acoplan el crecimiento celular y la división en levaduras
Paul Jorgensen1, Joy L Nishikawa, Bobby-Joe Breitkreutz
1Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
Science (New York, N.Y.)
|June 29, 2002
Resumen
Los investigadores identificaron 500 cepas de deleción de genes que afectan el tamaño celular en la levadura, revelando nuevos factores como Sfp1 que vinculan la biogénesis del ribosoma con el crecimiento celular y el tiempo de división.
Área de la Ciencia:
- Biología celular Biología celular.
- Genética La genética.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El control del tamaño celular es crucial para la progresión del ciclo celular.
- La levadura en ciernes (Saccharomyces cerevisiae) debe alcanzar un tamaño crítico antes de la división celular (inicio).
Objetivo del estudio:
- Para identificar los genes que regulan el tamaño crítico de la célula en el inicio.
- Comprender la red de factores que gobiernan el tamaño celular. homeostasis.
Principales métodos:
- Cribado sistemático de aproximadamente 6000 cepas de deleción genética de Saccharomyces cerevisiae.
- Análisis de las distribuciones de tamaño celular para identificar mutantes pequeños (whi) y grandes (lge).
- Análisis genético para descubrir redes reguladoras.
Principales resultados:
- Aproximadamente 500 cepas de deleción de genes exhibieron tamaños celulares anormales.
- Se identificaron reguladores clave del tamaño crítico de las células, incluyendo Sfp1, Sch9, Cdh1, Prs3 y Whi5.
- El descubrimiento de Sfp1 vincula la biogénesis del ribosoma con el tamaño de la célula mediante la regulación de al menos 60 genes de ensamblaje del ribosoma.
Conclusiones:
- El tamaño celular al inicio está controlado por una compleja red genética.
- La biogénesis del ribosoma es un determinante importante del tamaño celular.
- El crecimiento y la división celular están acoplados a través de mecanismos conservados.
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