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Recombinación: Células del bazo infectadas múltiples en pacientes con VIH

Andreas Jung¹, Reinhard Maier, Jean-Pierre Vartanian

¹Department of Virology, University of the Saarland, 66421 Homburg, Germany.

|July 12, 2002

Resumen

Los genomas del virus de la inmunodeficiencia humana (VIH) se recombinan fácilmente dentro de las células infectadas, generando una amplia diversidad genética. Esta alta tasa de recombinación y variación viral puede influir en las respuestas inmunes.

Área de la Ciencia:

Virología Virología.
Inmunología Inmunología.
Genética La genética.

Sus antecedentes:

El genoma del virus de la inmunodeficiencia humana (VIH) es conocido por su alta susceptibilidad a la recombinación.
Todavía no está claro si las recombinantes del VIH son raras o se generan con frecuencia pero no se detectan debido a la rápida rotación viral.

Objetivo del estudio:

Para investigar el grado de recombinación del VIH dentro de las células individuales.
Para cuantificar el número de provirus por célula y evaluar la variación genética.

Principales métodos:

Utilizó fluorescencia in situ hibridación (FISH) para contar los provirus en los esplenocitos de pacientes con VIH.
Se empleó la secuenciación de ADN de ADN proviral amplificado para detectar la recombinación y la variación genética.

Principales resultados:

Se identificó un promedio de tres a cuatro provirus del VIH por esplenocito.
Encontraron evidencia sustancial de numerosas recombinaciones y una amplia variación genética dentro de las células.

Conclusiones:

La recombinación del VIH ocurre con una alta frecuencia dentro de las células infectadas, lo que lleva a una significativa diversidad genética intracelular.
Esta extensa variación genética, a la vez que complica el análisis filogenético, podría potencialmente mejorar el reconocimiento inmune de las células infectadas.

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