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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Alternativa de empalme de pre-ARNm y expansión del proteoma en metazoanos
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Nature
|July 12, 2002
Resumen
El empalme alternativo de pre-ARNm genera diversidad de proteínas uniendo selectivamente elementos codificantes, eliminando intrones de los ARN precursores mensajeros. Este mecanismo contribuyó significativamente a la evolución de complejos proteomas en los metazoos.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
- Genética La genética.
Sus antecedentes:
- Los precursores de ARN mensajero de los eucariotas (pre-ARNm) contienen intrones no codificantes que interrumpen las secuencias codificadoras de proteínas.
- El empalme del pre-ARNm elimina los intrones y une los elementos codificantes para formar ARN mensajeros maduros (ARNm).
Objetivo del estudio:
- Investigar el papel del empalme alternativo del pre-ARNm en la generación de diversidad de proteínas.
- Comprender la importancia evolutiva del empalme alternativo en la expansión del proteoma de los metazoos.
Principales métodos:
- Análisis de los mecanismos de empalme del pre-ARNm.
- Genómica comparativa para estudiar la evolución del proteoma.
Principales resultados:
- El empalme alternativo de pre-ARNm permite la unión combinatoria de diferentes elementos codificadores de proteínas.
- Este proceso genera ARNm que codifican proteínas con funciones distintas, aumentando la diversidad de las proteínas.
Conclusiones:
- El empalme alternativo es un mecanismo clave para expandir el repertorio de proteínas codificadas por un genoma.
- La evolución del empalme alternativo jugó un papel crucial en la diversificación de los proteomas de los metazoos.
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01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:01pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:01Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...