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Funciones de la señalización del FGF desde la cresta ectodérmica apical en el desarrollo de las extremidades
Xin Sun1, Francesca V Mariani, Gail R Martin
1Department of Anatomy and Program in Developmental Biology, School of Medicine, University of California at San Francisco, 94143-0452, USA.
Nature
|August 2, 2002
Resumen
Los factores de crecimiento de los fibroblastos (FGF4 y FGF8) de la cresta ectodérmica apical son esenciales para el desarrollo de las extremidades al regular el número de células y la supervivencia. Su ausencia conduce al fracaso de la formación de extremidades en ratones.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- La cresta ectodérmica apical (AER) es crucial para el desarrollo de las extremidades.
- Los factores de crecimiento de los fibroblastos (FGF) son moléculas de señalización implicadas en el desarrollo embrionario.
Objetivo del estudio:
- Investigar las funciones específicas de la señalización de FGF4 y FGF8 de la AER en el desarrollo de las extremidades del ratón.
- Para entender cómo la señalización FGF influye en las poblaciones de células progenitoras y la formación de elementos esqueléticos.
Principales métodos:
- La inactivación de los genes Fgf4 y Fgf8 en las células AER o sus precursores durante el desarrollo de las extremidades del ratón.
- Análisis de los fenotipos esqueléticos en las extremidades de ratones mutantes.
- Modelación de los efectos mediados por el FGF en el tamaño de las extremidades y la supervivencia celular.
Principales resultados:
- FGF4 y FGF8 regulan la proliferación celular y la supervivencia dentro del desarrollo de la extremidad bud.
- Estos FGF son vitales para mantener las células progenitoras alejadas de la AER.
- La ausencia completa de actividad de FGF4 y FGF8 resulta en un desarrollo fallido de las extremidades.
Conclusiones:
- La señalización de FGF4 y FGF8 de la AER es indispensable para garantizar un número adecuado de células progenitoras para la formación de tejido esquelético y de otras extremidades.
- El estudio proporciona un modelo para la regulación mediada por FGF del tamaño de las yemas de las extremidades y la supervivencia celular durante el desarrollo.
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