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Biosíntesis de la canosamina: una fuente probable del átomo de nitrógeno de la vía del aminoshikimato
1Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA.
Journal of the American Chemical Society
|September 5, 2002
Resumen
La biosíntesis de la canosamina se identifica como la fuente del átomo de nitrógeno en la vía del aminoshikimato. Este estudio demuestra que el 6-fosfato de kanosamina es
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Las vías metabólicas.
- La biosíntesis microbiana es la biosíntesis microbiana.
Sus antecedentes:
- El origen del átomo de nitrógeno en la vía del aminoshikimato ha sido una cuestión sin resolver en la investigación bioquímica.
- Estudios previos habían indicado que el 6-fosfato de 3-amino-3-deoxy-D-fructosa era un precursor de la *Amycolatopsis mediterranei*.
Objetivo del estudio:
- Para investigar si el producto natural kanosamina sirve como un precursor de 3-amino-3-deoxy-D-fructosa 6-fosfato.
- Para dilucidar la fuente biosintética del átomo de nitrógeno incorporado en la vía del aminoshikimato.
Principales métodos:
- Síntesis quimoenzimática de la canosamina 6-fosfato.
- Incubación de 6-fosfato de kanosamina sintetizado con lisato libre de células de *A. mediterranei*, 5-fosfato de D-ribosa y fosfenolpiruvato.
- Incubación de UDP-glucosa con *A. mediterranei* lisato libre de células, glutamina y NAD para observar la formación de canosamina.
Principales resultados:
- Se observó la formación de 4-amino-3,4-dideoxy-D-arabino-heptulosónico ácido 7-fosfato y 3-amino-5-hidroxibenzoico ácido después de la incubación con kanosamina 6-fosfato.
- La canosamina se formó a partir de la UDP-glucosa en el lisado libre de células.
- Se confirmó la bioconversión del 6-fosfato de canosamina a los principales intermediarios de la vía del aminoshikimato.
Conclusiones:
- La bioconversión de la UDP-glucosa a la canosamina y la posterior conversión de la canosamina 6-fosfato a los intermediarios de la vía sugiere fuertemente la biosíntesis de la canosamina como fuente de nitrógeno.
- Este estudio resuelve la pregunta de larga data con respecto al origen del átomo de nitrógeno en la vía del aminoshikimato.
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