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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
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Un experimento de RMN para la medición precisa de las tasas de relajación del spin-lock heteronuclear

Dmitry M Korzhnev1, Nikolai R Skrynnikov, Oscar Millet

  • 1Protein Engineering Network Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada.

Journal of the American Chemical Society
|September 5, 2002
PubMed
Resumen

Los investigadores encontraron que las técnicas comunes de resonancia magnética nuclear (RMN) pueden sobreestimar las mediciones de dinámica de proteínas. Los métodos específicos de desacoplamiento de (1)H introducen errores en las mediciones de las tasas de relajación del marco rotativo heteronuclear (R(1) ((rho)).

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