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Una vía EGFR/Ebi/Sno promueve la expresión delta inactivando la represión Su(H) /SMRTER durante la señalización de
Leo Tsuda1, Raghavendra Nagaraj, S Lawrence Zipursky
1Department of Biological Chemistry and Department of Human Genetics, Molecular Biology Institute, Los Angeles, CA 90095, USA.
Cell
|September 17, 2002
Resumen
La activación del receptor del factor de crecimiento epidérmico (EGFR) en los ojos de Drosophila promueve la expresión Delta, iniciando una secuencia que influye en la diferenciación celular. Este proceso involucra proteínas específicas y depende de la actividad del proteasoma.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La señalización celular de las células.
Sus antecedentes:
- Las vías de Notch y del receptor del factor de crecimiento epidérmico (EGFR) son cruciales para la proliferación y diferenciación celular.
- El diálogo cruzado entre estas vías influye significativamente en las respuestas celulares.
- Comprender su interacción es clave para descifrar los procesos de desarrollo.
Objetivo del estudio:
- Para dilucidar el mecanismo que une la señalización de EGFR y Notch en el desarrollo del ojo de Drosophila.
- Para identificar los eventos secuenciales iniciados por la activación del EGFR que afectan la señalización posterior.
Principales métodos:
- Utilizado Drosophila melanogaster como un organismo modelo para el estudio de las vías de desarrollo.
- Investigó la expresión génica y las interacciones de proteínas relacionadas con la señalización EGFR y Notch.
- Se emplean ensayos de inhibición genética y proteasómica para comprender los mecanismos reguladores.
Principales resultados:
- La activación del EGFR en el desarrollo de los ojos de Drosophila conduce a la diferenciación de los fotorreceptores y la expresión del ligando Delta.
- Delta, inducido por el EGFR, posteriormente envía señales a las células vecinas, promoviendo su diferenciación en células cono.
- Las proteínas ebi y strawberry notch (sno) son esenciales para la transcripción delta dependiente del EGFR al modular la actividad del supresor de la pérdida de pelo (Su(H)).
- La derepresión transcripcional mediada por el EGFR es dependiente del proteasoma e implica la translocación citoplasmática SMRTER.
Conclusiones:
- Una cascada de señalización secuencial enlaza las vías EGFR y Notch en el desarrollo del ojo de Drosophila.
- La señalización del EGFR inicia un proceso que culmina en decisiones del destino celular mediadas por Notch.
- La interacción de ebi, sno, Su(H) y SMRTER es fundamental para regular este control transcripcional dependiente del EGFR.
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