Video Experimental Relacionado
Updated: May 28, 2026
13:02
The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
Published on: October 5, 2016
El resveratrol, una fitoalexina polifenólica presente en el vino tinto, mejora la expresión y la actividad de la
Thomas Wallerath1, Göran Deckert, Thomas Ternes
1Department of Pharmacology, Johannes Gutenberg University, Mainz, Germany.
Circulation
|September 25, 2002
Resumen
El resveratrol, que se encuentra en las uvas, aumenta la expresión endotelial de óxido nítrico sintasa (eNOS) y la producción de óxido nítrico (NO) en las células humanas. Este fitoestrógeno es un fitoestrógeno.
Área de la Ciencia:
- Ciencias Cardiovasculares Ciencias Cardiovasculares
- Biología Molecular Biología Molecular
- Farmacología Farmacología.
Sus antecedentes:
- Los estrógenos aumentan la regulación de la endotélica óxido nítrico sintetasa (eNOS) a través de la actividad promotora y la unión al factor de transcripción Sp1.
- El resveratrol, una fitoalexina de las uvas, actúa como un agonista del receptor de estrógeno.
- Este estudio investiga el impacto del resveratrol en la expresión de eNOS en las células endoteliales humanas.
Objetivo del estudio:
- Para determinar si el resveratrol influye en la expresión endotelial de la óxido nítrico sintetasa (eNOS).
- Para explorar el mecanismo por el cual el resveratrol afecta a eNOS.
- Evaluar los efectos a corto y largo plazo del resveratrol en la producción de óxido nítrico (NO).
Principales métodos:
- Las células endotelial de la vena umbilical humana (HUVEC) y las células EA.hy 926 se incubaron con resveratrol.
- Se midió la expresión de eNOS mRNA y proteínas, la actividad promotora y la producción de NO.
- Se utilizaron ensayos de desplazamiento de movilidad electroforética (EMSA) para evaluar la unión al factor de transcripción.
- El papel del receptor de estrógeno se investigó utilizando antagonistas.
Principales resultados:
- El resveratrol, dependiendo de la dosis y el tiempo, aumentó la regulación del ARNm eNOS (hasta 2,8 veces) y la expresión de proteínas.
- Se observó un aumento de la actividad del promotor eNOS, localizado en el proximal 263 bp.
- El resveratrol estabilizó el ARNm eNOS y aumentó la producción de NO, independientemente del antagonismo del receptor de estrógeno.
- La incubación a corto plazo también mejoró la producción de NO bioactivo.
Conclusiones:
- El resveratrol estimula la expresión y la actividad eNOS.
- Esta estimulación puede contribuir a los efectos protectores cardiovasculares del resveratrol.
- El mecanismo implica efectos directos en eNOS, no sólo a través del receptor de estrógeno.
Videos de Conceptos Relacionados
02:37Oxidation of Alcohols
In this lesson, the oxidation of alcohols is discussed in depth. The various reagents used for oxidation of primary and secondary alcohols are detailed, and their mechanism of action is provided.
The process of oxidation in a chemical reaction is observed in any of the three forms:
The process of oxidation in a chemical reaction is observed in any of the three forms:
01:24Peroxisomes
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
01:17Oxidation of Phenols to Quinones
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
01:20Radical Autoxidation
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
01:25Microbes in Beverage Production
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
01:27Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...