Video Experimental Relacionado
Updated: Jul 25, 2026
08:10
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
Published on: December 14, 2015
El modelado mediado por el erizo del embrión de mamífero requiere una función similar a la del transportador del
Yong Ma1, Alfrun Erkner, Ruoyu Gong
1Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Cell
|October 10, 2002
Resumen
La proteína DispA del ratón rescata la señalización de la mosca de la fruta Hedgehog. DispA del ratón es esencial para el desarrollo embrionario y la liberación de la proteína Hedgehog, lo que sugiere funciones conservadas en todas las especies.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- El gen despachado (disp) regula la señalización de largo alcance Hedgehog (Hh) en Drosophila.
- Las vías de señalización del erizo son cruciales para el desarrollo embrionario y la formación de patrones.
Objetivo del estudio:
- Para investigar la función de los homólogos murinos del gen Drosophila disp en la señalización de los mamíferos Hedgehog.
- Para aclarar el papel de mDispA en el desarrollo embrionario temprano del ratón y la señalización Sonic hedgehog (Shh).
- Para caracterizar la actividad bioquímica de las proteínas Disp en la liberación de proteínas de Hedgehog.
Principales métodos:
- Experimentos de rescate funcional en Drosophila utilizando homólogos murinos.
- Análisis de las actividades de patronización de Hh en embriones de ratón tempranos.
- Ensayos bioquímicos para medir la actividad de la proteína Disp en la liberación de Shh.
Principales resultados:
- El mDispA murino rescata la función del gen Drosophila disp.
- mDispA es esencial para todas las actividades de patronización Hh examinadas en los primeros embriones de ratón.
- Los fibroblastos que carecen de mDispA muestran un deterioro en la estimulación Shh de otras células.
- Un ensayo bioquímico demostró el papel de las proteínas Disp en la liberación de proteínas Hh solubles.
Conclusiones:
- El mDispA murino es un homólogo funcional de Drosophila disp y es crítico para la señalización de los mamíferos Hedgehog.
- Las proteínas de dispersión probablemente facilitan la liberación de la proteína Hedgehog a través de un mecanismo conservado con transportadores bacterianos y Patched.
- Comprender la función Disp proporciona información sobre los mecanismos conservados de la señalización del desarrollo.
Videos de Conceptos Relacionados
01:56Gastrulation
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:19Membrane Asymmetry Regulating Transporters
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
01:15Transcytosis of IgG
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...