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La activación directa de la transcripción de la ARN polimerasa III por el c-MycMyc

Natividad Gomez-Roman¹, Carla Grandori, Robert N Eisenman

¹Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow, G12 8QQ, UK.

|January 17, 2003

Resumen

El proto-oncogén c-Myc activa directamente la transcripción de la ARN polimerasa III (pol III) al unirse al TFIIIB. Este hallazgo sugiere un papel para la activación de pol III en c-Myc Myc.

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología celular Biología celular.
Oncología Oncología.

Sus antecedentes:

El proto-oncogén c-Myc es crucial para el crecimiento y la división celular.
La ARN polimerasa III (pol III) sintetiza ARN esenciales (ARNt, ARNr 5S) para la producción de proteínas en las células en crecimiento.

Objetivo del estudio:

Investigar el mecanismo por el cual c-Myc influye en la transcripción pol III.
Para determinar si c-Myc interactúa directamente con y regula la actividad de pol III.

Principales métodos:

Inmunoprecipitación de cromatina (ChIP) para detectar c-Myc endógeno que se une a los genes.
Pruebas bioquímicas para evaluar la interacción de c-Myc con los factores de transcripción.

Principales resultados:

Se encontró que c-Myc se une a TFIIIB, un factor de transcripción clave para el pol III.
c-Myc activa directamente la transcripción dependiente del pol III.
El c-Myc endógeno se localiza en los genes de tRNA y 5S rRNA en las células de mamíferos.

Conclusiones:

c-Myc activa directamente la transcripción de pol III a través del TFIIIB.
La activación de pol III por c-Myc puede contribuir a su papel en la estimulación del crecimiento celular.

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