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Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
La adhesión mediada por L-selectina del trofoblasto en la interfaz madre-fetal
Olga D Genbacev1, Akraporn Prakobphol, Russell A Foulk
1Departments of Stomatology, Anatomy, and Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA.
Science (New York, N.Y.)
|January 18, 2003
Resumen
La L-selectina del trofoblasto media en las interacciones cruciales con el útero, facilitando el establecimiento temprano del embarazo. Este sistema de adhesión, que involucra ligandos de selectina en las células uterinas y L-selectina en los trofoblastos, es vital para el éxito de la implantación y la placentación.
Área de la Ciencia:
- Biología de la reproducción Biología reproductiva.
- Mecanismos de adhesión celular Mecanismos de adhesión celular.
- Inmunología de la interfaz madre-fetal inmunología de la interfaz madre-fetal.
Sus antecedentes:
- La adhesión de los trofoblastos a la pared uterina es esencial para la implantación humana y la placentación.
- Los sistemas de adhesión de selectinas, típicamente involucrados en la captura de leucocitos, fueron investigados en la interfaz madre-feto.
- Comprender estas interacciones es clave para identificar los factores críticos para establecer el embarazo.
Objetivo del estudio:
- Investigar la expresión y la función de los sistemas de adhesión de selectinas en la interfaz madre-fetal durante el embarazo humano temprano.
- Determinar el papel de la L-selectina y sus ligandos en las interacciones trofoblasto-uterina.
- Para aclarar los mecanismos potenciales críticos para el éxito de la implantación humana.
Principales métodos:
- Análisis de la expresión del ligando de selectina en las células epiteliales uterinas humanas durante la ventana de receptividad.
- Detección de la expresión de L-selectina en trofoblastos humanos.
- Ensayos funcionales utilizando cuentas recubiertas con 6-sulfo sLe (x) para evaluar la unión a los trofoblastos.
- Evaluación del trophoblast que se une al epitelio luminal uterino en secciones de tejido.
Principales resultados:
- Las células epiteliales uterinas humanas aumentaron su regulación selectiva en ligandos basados en oligosacáridos durante la ventana de receptividad.
- Los trofoblastos humanos expresan la L-selectina.
- El sistema selectin ligando-receptor fue funcional, demostrado por la unión del epitelio uterino con el trofoblasto y el trofoblasto-perla.
- La L-selectina de trofoblastos fue identificada como un mediador de las interacciones uterinas.
Conclusiones:
- La L-selectina de trofoblastos juega un papel importante en la mediación de las interacciones con el útero.
- Este mecanismo de adhesión selectivamente mediado puede ser crítico para establecer el embarazo humano.
- Los hallazgos ponen de relieve una nueva vía de adhesión involucrada en la placentación temprana.
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