Video Experimental Relacionado
Updated: Jul 1, 2026
17:03
Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
Published on: March 24, 2010
Diez años de tensión: la mecánica del ADN de una sola molécula
Carlos Bustamante1, Zev Bryant, Steven B Smith
1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA. carlos@alice.berkeley.edu
Nature
|January 24, 2003
Resumen
Los investigadores ahora pueden medir las propiedades mecánicas del ADN manipulando moléculas individuales. Esto proporciona información sobre las interacciones ADN-proteína, las restricciones de la maquinaria celular y las fuerzas motoras dependientes del ADN.
Área de la Ciencia:
- La biofísica es la biofísica.
- Biología Molecular Biología Molecular
- La genómica es la genómica.
Sus antecedentes:
- La estructura fundamental del ácido desoxirribonucleico (ADN) se estableció hace décadas.
- Los avances recientes permiten la manipulación y la medición de propiedades mecánicas de moléculas de ADN individuales.
Videos de Conceptos Relacionados
01:16The DNA Helix
Overview
00:58DNA Packaging
Overview
01:16The DNA Helix
Overview
00:55DNA Helicases
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
02:02DNA Topoisomerases
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
01:07The DNA Helix
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...