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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

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Here, we describe the assembly of RNA polymerase II (Pol II) elongation complexes requiring only short synthetic DNA and RNA oligonucleotides and purified Pol II. These complexes are useful for studying mechanisms underlying co-transcriptional processing of transcripts associated with the Pol II elongation...
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An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector10:13

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

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This protocol describes a method to achieve stable integration of a gene of interest into the human genome by the transcriptionally regulated Sleeping Beauty system. Preparation of the integration of defective lentiviral vectors, the in vitro transduction of human cells, and the molecular assay on transduced cells are...
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

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Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.
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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp111:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

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With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter...
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Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration10:43

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

7.1K
MRP4 regulates various cyclic nucleotide-dependent signaling events including a recently elucidated role in cell migration. We describe a direct, but multifaceted approach to unravel the downstream molecular targets of MRP4 resulting in identification of a unique MRP4 interactome that plays key roles in the fine-tuned regulation of fibroblast...
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High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’...
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Video Experimental Relacionado

Updated: Jan 29, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

10.2K

El código digital del ADN.

Leroy Hood1, David Galas

  • 1Institute for Systems Biology, 4225 Roosevelt Way NE, Seattle, Washington 98105, USA. lhood@systemsbiology.org

Nature
|January 24, 2003
PubMed
Resumen

El descubrimiento de la estructura del ADN revolucionó la biología, revelando su naturaleza digital y complementaria. Esta comprensión sustenta la genómica y las redes reguladoras de genes, viendo la biología como una ciencia de la información.

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • La genómica es la genómica.
  • La bioinformática es la bioinformática.

Sus antecedentes:

  • La aclaración de la estructura del ADN marcó un cambio de paradigma en las ciencias biológicas.
  • Este descubrimiento allanó el camino para el Proyecto del Genoma Humano y la biología molecular avanzada.
  • Estableció una nueva perspectiva de la biología como una ciencia de la información.

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