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Videos de Conceptos Relacionados

Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
What are Membranes?01:24

What are Membranes?

A cell's plasma membrane demarcates the cell's borders and determines the nature of its interaction with the environment. Cells exclude certain substances, take in others, and excrete some others in controlled quantities. The plasma membrane must be flexible to allow certain cells, such as red and white blood cells, to change their shape while passing through narrow capillaries. These are the more obvious plasma membrane functions. In addition, the plasma membrane's surface carries markers that...

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Video Experimental Relacionado

Updated: May 11, 2026

Cell Electrofusion Visualized with Fluorescence Microscopy
05:02

Cell Electrofusion Visualized with Fluorescence Microscopy

Published on: July 2, 2010

La fusión de la membrana por fusión de la membrana.

Reinhard Jahn1, Thorsten Lang, Thomas C Südhof

  • 1Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany. rjahn@gwdg.de

Cell
|February 26, 2003
PubMed
Resumen
Este resumen es generado por máquina.

La fusión de membranas fusiona las bicapas lipídicas, un proceso biológico vital. Diversas proteínas catalizan esta función celular esencial al unir las membranas para iniciar la mezcla de lípidos.

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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

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Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
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Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Published on: April 5, 2018

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Cell Electrofusion Visualized with Fluorescence Microscopy
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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

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Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.
11:10

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Published on: April 5, 2018

Área de la Ciencia:

  • Biología celular Biología celular.
  • La bioquímica es la bioquímica.

Sus antecedentes:

  • La fusión de membranas es un proceso biológico fundamental en el que dos bicapas lipídicas se fusionan en una.
  • Este proceso es crucial para varias funciones celulares, incluyendo el transporte y la señalización.
  • Diversas proteínas catalizan la fusión de la membrana, asegurando la especificidad y la eficiencia.

Objetivo del estudio:

  • Explorar los principios comunes y los diversos mecanismos de la fusión de membrana catalizada por proteínas.
  • Comprender cómo las proteínas median el reconocimiento de la membrana, la proximidad y la desestabilización para la fusión de dos capas.
  • Para resaltar las funciones reguladoras de los complejos de proteínas en los eventos de fusión intracelular.

Principales métodos:

  • Análisis comparativo de eventos conocidos de fusión de membrana.
  • Revisión de las estructuras y funciones de las proteínas involucradas en la fusión.
  • Examen de las interacciones lípido-proteína durante la fusión de membranas.

Principales resultados:

  • Las proteínas de fusión de membrana comparten principios funcionales comunes a pesar de sus diversas estructuras.
  • Las proteínas median el reconocimiento inicial de la membrana y acercan las dos capas.
  • La desestabilización de la interfaz lípido/agua y la mezcla de lípidos son resultados clave de la fusión.
  • La fusión intracelular puede implicar ensamblajes complejos de proteínas para una regulación precisa.

Conclusiones:

  • Las máquinas de fusión celular, aunque adaptadas a necesidades específicas, funcionan con principios universales.
  • La catálisis de proteínas es esencial para regular el proceso fundamental de la fusión de la membrana.
  • La comprensión de estos mecanismos proporciona una visión de la organización celular y la función.