Video Experimental Relacionado
Updated: Jul 11, 2026
11:40
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
La activación de la función lisosomal durante la maduración de las células dendríticas.
E Sergio Trombetta1, Melanie Ebersold, Wendy Garrett
1Department of Cell Biology and Department of Immunobiology, Ludwig Institute for Cancer Research, Yale University School of Medicine, 333 Cedar Street, Post Office Box 208002, New Haven, CT 06520-8002, USA.
Science (New York, N.Y.)
|March 1, 2003
Resumen
Las células dendríticas (CD) mejoran la presentación de antígenos durante la maduración mediante la activación de la función lisosomal. Esto aumenta la formación del complejo péptido-MHC clase II, crucial para la estimulación de las células T.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología celular Biología celular.
Sus antecedentes:
- Las células dendríticas (CD) se diferencian de las células inmaduras de captura de antígenos a las células maduras estimulantes de células T tras la estimulación.
- Durante la maduración, los CD mejoran su capacidad para formar complejos de peptido-complejo mayor de histocompatibilidad (MHC) de clase II.
Objetivo del estudio:
- Esclarecer el mecanismo detrás de la formación del complejo de clase II de péptido-MHC mejorado durante la maduración de células dendríticas.
Principales métodos:
- Investigó el papel de la función lisosomal y la actividad de la bomba de protones vacuolar en la maduración de las células dendríticas.
- Se evaluó la degradación de antígenos y la eficiencia de carga de péptidos en DC inmaduros versus maduros.
Principales resultados:
- Los DC inmaduros exhibieron una lenta degradación de antígenos y una carga ineficiente de péptidos.
- La maduración de CC activó la bomba de protones vacuolar, aumentando la acidificación lisosomal y la proteólisis.
- Esta mayor actividad lisosómica facilitó la formación eficiente de complejos péptido-MHC clase II.
Conclusiones:
- La función lisosomal está regulada en el desarrollo en DCs para un procesamiento eficiente de antígenos.
- La activación de las vías lisosomales es un mecanismo clave que impulsa la maduración de la DC y la estimulación de las células T.
Videos de Conceptos Relacionados
01:28Maturation of Endosomes
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
01:31Lysosomes
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
01:36Delivery Pathways to the Lysosome
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
01:27Autophagy
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
01:22Lysosomal Hydrolases
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
01:31Lysosomes
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...